Frequency and phenotype of EHV-1 specific, IFN-γ synthesising lymphocytes in ponies:: The effects of age, pregnancy and infection

被引：25

作者：

Paillot, Rornain ^{[1
]}

Daly, Janet M. ^{[1
]}

Luce, Richard ^{[1
]}

Montesso, Fernando ^{[1
]}

Davis-Poynter, Nick ^{[1
]}

Hannant, Duncan ^{[1
]}

Kydd, Julia H. ^{[1
]}

机构：

[1] Anim Hlth Trust, Ctr Prevent Med, Newmarket CB8 7UU, Suffolk, England

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2007年 / 31卷 / 02期

关键词：

equine IFN gamma; equine herpesvirus 1; immunity; age; foal; pregnancy; Cd8 T lymphocyte; kinetics;

D O I：

10.1016/j.dci.2006.05.010

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Equine herpesvirus-1 (EHV-1) infects horses, causing acute respiratory disease, neurological signs, and is also a leading cause of abortion. Protection from EHV-1 infection and disease depends on both humoral (virus neutralising antibody) and cellular (mainly cytotoxic T lymphocytes, CTL) immune responses. CTL activity after EHV-1 infection has been extensively investigated and is closely associated with an alternative measure of cell mediated immunity (CMI), interferongamma (IFN-gamma) synthesis. This study investigates EHV-1-specific IFN-gamma synthesising cells in potentially immunocompromised horses; foals, pregnant mares and aged animals, after field or experimental infection with EHV-1. In foals and pregnant mares, the kinetics after experimental infection were similar and the phenotype of IFN-gamma(+) synthesising cells after EHV-1 stimulation was mainly CD8 alpha(+). In contrast, in samples collected from primed healthy ponies exposed to EHV-1 several months previously or in old ponies (28 years old), the majority of EHV-1-specific IFN-gamma(+) lymphocytes expressed a CD5(+), CD8 alpha(-) phenotype. This study highlights the complexity of the relationship between EHV-1, a common pathogen in horses, and the virus-specific cellular immune response as measured using IFN-gamma synthesis. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：202 / 214

页数：13

共 44 条

[21] DISTRIBUTION OF EQUID HERPESVIRUS-1 (EHV-1) IN RESPIRATORY-TRACT ASSOCIATED LYMPHOID-TISSUE - IMPLICATIONS FOR CELLULAR-IMMUNITY [J].

KYDD, JH ;

SMITH, KC ;

HANNANT, D ;

LIVESAY, GJ ;

MUMFORD, JA .

EQUINE VETERINARY JOURNAL, 1994, 26 (06) :470-473

[22] Pre-infection frequencies of equine herpesvirus-1 specific, cytotoxic T lymphocytes correlate with protection against abortion following experimental infection of pregnant mares [J].

Kydd, JH ;

Wattrang, E ;

Hannant, D .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2003, 96 (3-4) :207-217

[23] Positive selection of EqCD8(+) precursors increases equine lymphokine-activated killing [J].

Lunn, DP ;

Schram, BR ;

Vagnoni, KE ;

Schobert, CS ;

Horohov, DW ;

Ginther, OJ .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1996, 53 (1-2) :1-13

[24] Comparative T-cell responses during pregnancy in large animals and humans [J].

Meeusen, ENT ;

Bischof, RJ ;

Lee, CS .

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2001, 46 (02) :169-179

[25]

MUMFORD JA, 1994, P 7 INT C EQ INF DIS, P261

[26] Determination of equid herpesvirus 1-specific, CD8+, cytotoxic T lymphocyte precursor frequencies in ponies [J].

O'Neill, T ;

Kydd, JH ;

Allen, GP ;

Wattrang, E ;

Mumford, JA ;

Hannant, D .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1999, 70 (1-2) :43-54

[27]

ONEILL T, 1995, THESIS OPEN U, P300

[28] Characterisation of CTL and IFN-γ synthesis in ponies following vaccination with a NYVAC-based construct coding for EHV-1 immediate early gene, followed by challenge infection [J].

Paillot, R ;

Ellis, SA ;

Daly, JM ;

Audonnet, JC ;

Minke, JM ;

Davis-Poynter, N ;

Hannant, D ;

Kydd, JH .

VACCINE, 2006, 24 (10) :1490-1500

[29] Equine interferon gamma synthesis in lymphocytes after in vivo infection and in vitro stimulation with EHV-1 [J].

Paillot, R ;

Daly, JM ;

Juillard, V ;

Minke, JM ;

Hannant, D ;

Kydd, JH .

VACCINE, 2005, 23 (36) :4541-4551

[30]

PAILLOT R, 2006, IN PRESS VET IMMUNOL

← 1 2 3 4 5 →