Integrated Transcriptomic, Phenotypic, and Functional Study Reveals Tissue-Specific Immune Properties of Mesenchymal Stromal Cells

被引：58

作者：

Menard, Cedric ^{[1
,2
]}

Dulong, Joelle ^{[1
,2
]}

Roulois, David ^{[1
]}

Hebraud, Benjamin ^{[3
]}

Verdiere, Lea ^{[1
]}

Pangault, Celine ^{[1
,4
]}

Sibut, Vonick ^{[1
,2
]}

Bezier, Isabelle ^{[1
,2
]}

Bescher, Nadege ^{[1
,2
]}

Monvoisin, Celine ^{[1
]}

Gadelorge, Melanie ^{[3
]}

Bertheuil, Nicolas ^{[2
,5
]}

Flecher, Erwan ^{[6
]}

Casteilla, Louis ^{[3
]}

Collas, Philippe ^{[7
]}

Sensebe, Luc ^{[3
]}

Bourinp, Philippe ^{[8
]}

Espagnolle, Nicolas ^{[3
]}

Tarte, Karin ^{[1
,2
]}

机构：

[1] Univ Rennes, Etab Francais Sang Bretagne, INSERM, UMR 1236, Rennes, France

[2] CHU Rennes, SITI Lab, Etab Francais Sang Bretagne, Rennes, France

[3] Univ Toulouse, Etab Francais Sang Occitanie EFS, Inserm 1031, Natl Vet Sch Toulouse ENVT,STROMALab,CNRS ERL531, Toulouse, France

[4] CHU Rennes, Pole Biol, Rennes, France

[5] CHU Rennes, Dept Plast Surg, Rennes, France

[6] CHU Rennes, Dept Thorac & Cardiac Surg, Rennes, France

[7] Univ Oslo, Fac Med, Inst Basic Med Sci, Dept Mol Med, Oslo, Norway

[8] Cell Easy, Pl Pierre Potier, Toulouse, France

来源：

STEM CELLS | 2020年 / 38卷 / 01期

关键词：

BONE-MARROW; INDOLEAMINE 2,3-DIOXYGENASE; ADIPOSE-TISSUE; STEM-CELLS; DIFFERENTIATION; COMPLEMENT; MOUSE;

D O I：

10.1002/stem.3077

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Clinical-grade mesenchymal stromal cells (MSCs) can be expanded from bone marrow and adipose tissue to treat inflammatory diseases and degenerative disorders. However, the influence of their tissue of origin on their functional properties, including their immunosuppressive activity, remains unsolved. In this study, we produced paired bone marrow-derived mesenchymal stromal cell (BM-MSC) and adipose-derived stromal cell (ASC) batches from 14 healthy donors. We then compared them using transcriptomic, phenotypic, and functional analyses and validated our results on purified native MSCs to infer which differences were really endowed by tissue of origin. Cultured MSCs segregated by their tissue of origin, based on their gene expression profile, were analyzed using differential expression and weighted gene coexpression network analysis. This translated into distinct immune-related gene signatures, phenotypes, and functional cell interactions. Importantly, sorted native BM-MSCs and ASCs essentially displayed the same distinctive patterns as their in vitro-expanded counterparts. As a whole, ASCs exhibited an immune profile consistent with a stronger inhibition of immune response and a lower immunogenicity, supporting the use of adipose tissue as a valuable source for clinical applications. Stem Cells 2019

引用

页码：146 / 159

页数：14