Tracking the secondary photodynamics of the green/red cyanobacteriochrome RcaE from Fremyella diplosiphon

被引：7

作者：

Chang, Che-Wei ^{[1
]}

Gottlieb, Sean M. ^{[1
]}

Rockwell, Nathan C. ^{[2
]}

Martin, Shelley S. ^{[2
]}

Lagarias, J. Clark ^{[2
]}

Larsen, Delmar S. ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

[2] Univ Calif Davis, Dept Mol & Cell Biol, Davis, CA 95616 USA

来源：

CHEMICAL PHYSICS LETTERS | 2016年 / 644卷

基金：

美国能源部;

关键词：

GAF DOMAIN; PHOTOCONVERSION MECHANISM; PRIMARY PHOTOCHEMISTRY; PHYTOCHROME; ANPIXJ; SPECTROSCOPY; DYNAMICS; PHOTOISOMERIZATION; PHOTORECEPTOR; INHOMOGENEITY;

D O I：

10.1016/j.cplett.2015.11.059

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Cyanobacteriochrome RcaE regulates Type III complementary chromatic adaption in the cyanobacterium Fremyella diplosiphon by photoswitching between a green-absorbing dark state (P-15z(g)) and red-absorbing photoproduct (P-15E(r)). Ultrafast photodynamics of RcaE involve tautomerization of the bilin chromophore, inhomogeneity, and the generation of three primary photointermediates in the forward reaction (Lumi-G(0), Lumi-G(r), and Lumi-G(f)). The secondary photodynamics reported here show that only Lumi-G(0) evolves to P-15E(r) via spectrally similar Meta-G(o1) and Meta-G(o2) intermediates, with a protonation reaction occurring at the final step on the millisecond timescale. Reverse reaction dynamics were characterized and reveal an unusually long-lived Lumi-R-f photoproduct and a blue-shifted Meta-R-y intermediate. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：225 / 230

页数：6

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