Defective myogenic differentiation of human rhabdomyosarcoma cells is characterized by sialidase Neu2 loss of expression

被引：7
作者：
Stoppani, Elena [1 ]
Rossi, Stefania [1 ]
Marchesini, Sergio [1 ]
Preti, Augusto [1 ]
Fanzani, Alessandro [1 ]
机构：
[1] Univ Brescia, Biochem Unit, Dept Biomed Sci & Biotechnol, I-25123 Brescia, Italy
来源：
CELL BIOLOGY INTERNATIONAL | 2009年 / 33卷 / 09期
关键词：
Neu2; Sialidases; Rhabdomyosarcoma; p38; Follistatin; CYTOSOLIC SIALIDASE; TERMINAL DIFFERENTIATION; MYOBLAST DIFFERENTIATION; GENE; HYPERTROPHY; ACTIVATION; INDUCTION; PROGRAM; CLONING; FUSION;
D O I：
10.1016/j.cellbi.2009.06.005
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Sialidase Neu2 is a glycohydrolytic enzyme whose tissue distribution has been detected principally in differentiated skeletal muscle. In this study we show that Neu2 expression is absent in different embryonal and alveolar human tumor rhabdomyosarcoma (RMS) cells, which are genetically committed myoblasts characterized by delayed differentiation. Forced myogenic differentiation of an embryonal RMS cell line, as obtained via pharmacological and genetic p38 activation or via follistatin overexpression, was characterized by Neu2 loss of expression despite the significant rise of different muscle-specific markers, suggesting therefore that the defective myogenic program of RMS cells is accompanied by Neu2 suppression. (C) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：1020 / 1025
页数：6
相关论文
共 22 条
[1]   TPA-INDUCED DIFFERENTIATION OF HUMAN RHABDOMYOSARCOMA CELLS - EXPRESSION OF THE MYOGENIC REGULATORY FACTORS [J].
BOUCHE, M ;
SENNI, MI ;
GROSSI, AM ;
ZAPPELLI, F ;
POLIMENI, M ;
ARNOLD, HH ;
COSSU, G ;
MOLINARO, M .
EXPERIMENTAL CELL RESEARCH, 1993, 208 (01) :209-217
[2]   Overexpression of cytosolic sialidase Neu2 induces myoblast differentiation in C2C12 cells [J].
Fanzani, A ;
Giuliani, R ;
Colombo, F ;
Zizioli, D ;
Presta, M ;
Preti, A ;
Marchesini, S .
FEBS LETTERS, 2003, 547 (1-3) :183-188
[3]   The enzymatic activity of sialidase Neu2 is inversely regulated during in vitiro myoblast hypertrophy and atrophy [J].
Fanzani, Alessandro ;
Giuliani, Roberta ;
Colombo, Francesca ;
Rossi, Stefania ;
Stoppani, Elena ;
Martinet, Wim ;
Preti, Augusto ;
Marchesini, Sergio .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 370 (02) :376-381
[4]   Insulin-like growth factor 1 signaling regulates cytosolic sialidase Neu2 expression during myoblast differentiation and hypertrophy [J].
Fanzani, Alessandro ;
Colombo, Francesca ;
Giuliani, Roberta ;
Preti, Augusto ;
Marchesini, Sergio .
FEBS JOURNAL, 2006, 273 (16) :3709-3721
[5]   FUSION OF A FORK HEAD DOMAIN GENE TO PAX3 IN THE SOLID TUMOR ALVEOLAR RHABDOMYOSARCOMA [J].
GALILI, N ;
DAVIS, RJ ;
FREDERICKS, WJ ;
MUKHOPADHYAY, S ;
RAUSCHER, FJ ;
EMANUEL, BS ;
ROVERA, G ;
BARR, FG .
NATURE GENETICS, 1993, 5 (03) :230-235
[6]   Deacetylase inhibitors increase muscle cell size by promoting myoblast recruitment and fusion through induction of follistatin [J].
Iezzi, S ;
Di Padova, M ;
Serra, C ;
Caretti, G ;
Simone, C ;
Maklan, E ;
Minetti, G ;
Zhao, P ;
Hoffman, EP ;
Puri, PL ;
Sartorelli, V .
DEVELOPMENTAL CELL, 2004, 6 (05) :673-684
[7]   Myostatin inhibits rhabdomyosarcoma cell proliferation through an Rb-independent pathway [J].
Langley, B ;
Thomas, M ;
McFarlane, C ;
Gilmour, S ;
Sharma, M ;
Kambadur, R .
ONCOGENE, 2004, 23 (02) :524-534
[8]   HUMAN CHROMOSOME-11 CONTAINS 2 DIFFERENT GROWTH SUPPRESSOR GENES FOR EMBRYONAL RHABDOMYOSARCOMA [J].
LOH, WE ;
SCRABLE, HJ ;
LIVANOS, E ;
ARBOLEDA, MJ ;
CAVENEE, WK ;
OSHIMURA, M ;
WEISSMAN, BE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) :1755-1759
[9]  
LOLLINI PL, 1991, INVAS METAST, V11, P116
[10]   PKCα-mediated ERK, JNK and p38 activation regulates the myogenic program in human rhabdomyosarcoma cells [J].
Mauro, A ;
Ciccarelli, C ;
De Cesaris, P ;
Scoglio, A ;
Bouché, M ;
Molinaro, M ;
Aquino, A ;
Zani, BM .
JOURNAL OF CELL SCIENCE, 2002, 115 (18) :3587-3599
123