Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia gladiata seeds

被引：13

作者：

Moreno, FBMB

Delatorre, P

Freitas, BT

Rocha, BAM

Souza, EP

Facó, F

Canduri, F

Cardoso, ALH

Freire, VN

Lima, JL

Sampaio, AH

Calvete, JJ

De Azevedo, WF

Cavada, BS ^{[1
]}

机构：

[1] Univ Fed Ceara, Dept Bioquim & Biol Mol, BR-60451970 Fortaleza, Ceara, Brazil

[2] CSIC, Inst Biomed Valencia, Valencia 46010, Spain

[3] Univ Fed Ceara, Dept Engn Pesca, BR-60451970 Fortaleza, Ceara, Brazil

[4] Univ Fed Pernambuco, Lab Imunopatol Keizo Asami, BR-50670901 Recife, PE, Brazil

[5] Univ Fed Ceara, Dept Fis, BR-60451970 Fortaleza, Ceara, Brazil

[6] FAPESP, CEPID, Ctr Appl Toxinol, BR-05503900 Sao Paulo, Brazil

[7] UNESP, Dept Fis, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil

[8] Univ Reg Cariri, Dept Ciencias Biol, Grp Quim Biol, BR-63105000 Crato, Ceara, Brazil

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷

关键词：

D O I：

10.1107/S0907444904014489

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The seed lectin from Canavalia gladiata was purified and crystallized. Orthorhombic crystals belonging to space group C222(1) grew within three weeks at 293 K using the hanging-drop vapour-diffusion method. Using synchrotron X-ray radiation, a complete structural data set was collected at 2.3 Angstrom resolution. The preliminary crystal structure of the lectin, determined by molecular replacement, had a correlation coefficient of 0.569 and an R factor of 0.412.

引用

页码：1493 / 1495

页数：3

共 27 条

[1] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[2] HUMAN LYMPHOCYTE STIMULATION BY LEGUME LECTINS FROM THE DIOCLEAE TRIBE [J].

BARRALNETTO, M ;

SANTOS, SB ;

BARRAL, A ;

MOREIRA, LIM ;

SANTOS, CF ;

MOREIRA, RA ;

OLIVEIRA, JTA ;

CAVADA, BS .

IMMUNOLOGICAL INVESTIGATIONS, 1992, 21 (04) :297-303

[3] RAT PAW EDEMA AND LEUKOCYTE IMMIGRATION INDUCED BY PLANT-LECTINS [J].

BENTO, CAM ;

CAVADA, BS ;

OLIVEIRA, JTA ;

MOREIRA, RA ;

BARJAFIDALGO, C .

AGENTS AND ACTIONS, 1993, 38 (1-2) :48-54

[4] The Protein Data Bank [J].

Berman, HM ;

Westbrook, J ;

Feng, Z ;

Gilliland, G ;

Bhat, TN ;

Weissig, H ;

Shindyalov, IN ;

Bourne, PE .

NUCLEIC ACIDS RESEARCH, 2000, 28 (01) :235-242

[5]

BRUNGER AT, 1992, XPLOR VERSION 3 1 SY

[6] Molecular characterization and crystallization of Diocleinae lectins [J].

Calvete, JJ ;

Thole, HH ;

Raida, M ;

Urbanke, C ;

Romero, A ;

Grangeiro, TB ;

Ramos, MV ;

da Rocha, IMA ;

Guimaraes, FN ;

Cavada, BS .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1999, 1430 (02) :367-375

[7] Purification and partial characterization of a lectin from Canavalia grandiflora Benth. seeds [J].

Ceccatto, VM ;

Cavada, BS ;

Nunes, EP ;

Nogueira, NAP ;

Grangeiro, MB ;

Moreno, FBMB ;

Teixeira, EH ;

Sampaio, AH ;

Alves, MAO ;

Ramos, MV ;

Calvete, JJ ;

Grangeiro, TB .

PROTEIN AND PEPTIDE LETTERS, 2002, 9 (01) :67-73

[8] Amino acid sequence and tertiary structure of Cratylia mollis seed lectin [J].

de Souza, GA ;

Oliveira, PSL ;

Trapani, S ;

Santos, ACO ;

Rosa, JC ;

Laure, HJ ;

Faça, VM ;

Correia, MTS ;

Tavares, GA ;

Oliva, G ;

Coelho, LCBB ;

Greene, LJ .

GLYCOBIOLOGY, 2003, 13 (12) :961-972

[9] Literature review of an underutilized legume:: Canavalia gladiata L. [J].

Ekanayake, S ;

Jansz, ER ;

Nair, BM .

PLANT FOODS FOR HUMAN NUTRITION, 2000, 55 (04) :305-321

[10] HISTAMINE-RELEASE INDUCED BY GLUCOSE (MANNOSE)-SPECIFIC LECTINS ISOLATED FROM BRAZILIAN BEANS - COMPARISON WITH CONCANAVALIN-A [J].

GOMES, JC ;

FERREIRA, RR ;

CAVADA, BS ;

MOREIRA, RA ;

OLIVEIRA, JTA .

AGENTS AND ACTIONS, 1994, 41 (3-4) :132-135

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