Databases for technical aspects of immunohistochemistry

被引：52

作者：

Furukawa, Satoshi ^{[1
,2
]}

Nagaike, Mika ^{[1
,3
]}

Ozaki, Kiyokazu ^{[1
,4
]}

机构：

[1] Nissan Chem Ind Co Ltd, Conference Expt Anim Histopathol, 1470 Shiraoka, Shiraoka, Saitama 3490294, Japan

[2] Nissan Chem Ind Co Ltd, Biol Res Labs, 1470 Shiraoka, Shiraoka, Saitama 3490294, Japan

[3] Ishihara Sangyo Kaisha Ltd, Regulatory Affairs Div, Biosci Sales & Mkt, 2-3-1 Nishishibukawa, Kusatsu, Shiga 5250025, Japan

[4] Setsunan Univ, Fac Pharmaceut Sci, Pathol Lab, 45-1 Nagaotohge Cho, Hirakata, Osaka 5730101, Japan

来源：

JOURNAL OF TOXICOLOGIC PATHOLOGY | 2017年 / 30卷 / 01期

关键词：

antibody; immunohistochemistry; toxicological pathology;

D O I：

10.1293/tox.2016-0047

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

With the aims of sharing information about the technical aspects of immunohistochemistry (IHC) and making it possible to make a suitable choice of antibody for histopathological examination, this technical report describes the results of a questionnaire administered during the period of 2014 to 2015 to members of the Conference on Experimental Animal Histopathology. It also describes the immunological properties of primary antibodies (clone, supplier, catalog number, species reactivity, etc.) and the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, secondary antibody information, etc.) for a total number of 733 primary antibodies (425 kinds of primary antibody).

引用

页码：79 / 107

页数：29