Porous Porphyrin-Based Organosilica Nanoparticles for NIR Two-Photon Photodynamic Therapy and Gene Delivery in Zebrafish

被引：66

作者：

Jimenez, Chiara Mauriello ^{[1
]}

Aggad, Dina ^{[2
]}

Croissant, Jonas G. ^{[3
]}

Tresfield, Karen ^{[2
]}

Laurencin, Danielle ^{[1
]}

Berthomieu, Dorothee ^{[1
]}

Cubedo, Nicolas ^{[4
]}

Rossel, Mireille ^{[4
]}

Alsaiari, Shahad ^{[3
]}

Anjum, Dalaver H. ^{[5
]}

Sougrat, Rachid ^{[5
]}

Roldan-Gutierrez, Manuel A. ^{[5
]}

Richeter, Sebastien ^{[1
]}

Oliviero, Erwan ^{[1
]}

Raehm, Laurence ^{[1
]}

Charnay, Clarence ^{[1
]}

Cattoen, Xavier ^{[6
,7
]}

Clement, Sebastien ^{[1
]}

Man, Michel Wong Chi ^{[1
]}

Maynadier, Marie ^{[8
]}

Chaleix, Vincent ^{[9
]}

Sol, Vincent ^{[9
]}

Garcia, Marcel ^{[2
]}

Gary-Bobo, Magali ^{[2
]}

Khashab, Niveen M. ^{[3
]}

Bettache, Nadir ^{[2
]}

Durand, Jean-Olivier ^{[1
]}

机构：

[1] Inst Charles Gerhardt Montpellier, UMR CNRS UM ENSCM Cc 1701 5253, Pl Eugene Bataillon, F-34095 Montpellier 05, France

[2] UM Fac Pharm, UMR CNRS 5247, Inst Biomol Max Mousseron, 15 Ave Charles Flahault, F-34093 Montpellier 05, France

[3] King Abdullah Univ Sci & Technol, Adv Membranes & Porous Mat Ctr, Smart Hybrid Mat Lab, Thuwal 239556900, Saudi Arabia

[4] Univ Montpellier, MMDN, INSERM U1198, Cc 105,Pl Eugene Bataillon, F-34095 Montpellier 05, France

[5] King Abdullah Univ Sci & Technol, Imaging & Characterizat Lab, Thuwal 239556900, Saudi Arabia

[6] CNRS, Inst Neel, F-38042 Grenoble, France

[7] Univ Grenoble Alpes, F-38042 Grenoble, France

[8] NanoMedSyn, 15 Ave Charles Flahault, F-34000 Montpellier 05, France

[9] Univ Limoges, Lab PEIRENE, EA 7500, F-87060 Limoges, France

来源：

ADVANCED FUNCTIONAL MATERIALS | 2018年 / 28卷 / 21期

关键词：

nucleic acid delivery; organosilica nanoparticles; porphyrins; two-photon photodynamic therapy; zebrafish embryos; PERIODIC MESOPOROUS ORGANOSILICAS; DRUG-DELIVERY; HYBRIDIZATION; FRAMEWORK; CELLS;

D O I：

10.1002/adfm.201800235

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Periodic mesoporous organosilica nanoparticles emerge as promising vectors for nanomedicine applications. Their properties are very different from those of well-known mesoporous silica nanoparticles as there is no silica source for their synthesis. So far, they have only been synthesized from small bis-silylated organic precursors. However, no studies employing large stimuli-responsive precursors have been reported on such hybrid systems yet. Here, the synthesis of porphyrin-based organosilica nanoparticles from a large octasilylated metalated porphyrin precursor is described for applications in near-infrared two-photon-triggered spatiotemporal theranostics. The nanoparticles display unique interconnected large cavities of 10-80 nm. The framework of the nanoparticles is constituted with J-aggregates of porphyrins, which endows them with two-photon sensitivity. The nanoparticle efficiency for intracellular tracking is first demonstrated by the in vitro near-infrared imaging of breast cancer cells. After functionalization of the nanoparticles with aminopropyltriethoxysilane, two-photon-excited photodynamic therapy in zebrafish is successfully achieved. Two-photon photochemical internalization in cancer cells of the nanoparticles loaded with siRNA is also performed for the first time. Furthermore, siRNA targeting green fluorescent protein complexed with the nanoparticles is delivered in vivo in zebrafish embryos, which demonstrates the versatility of the nanovectors for biomedical applications.

引用

页数：12

共 28 条

[1] A Zinc Phthalocyanine Based Periodic Mesoporous Organosilica Exhibiting Charge Transfer to Fullerenes [J].