Epiblast Formation by TEAD-YAP-Dependent Expression of Pluripotency Factors and Competitive Elimination of Unspecified Cells

被引：103

作者：

Hashimoto, Masakazu ^{[1
]}

Sasaki, Hiroshi ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Frontier Biosci, Lab Embryogenesis, Suita, Osaka 5650871, Japan

来源：

DEVELOPMENTAL CELL | 2019年 / 50卷 / 02期

关键词：

HIPPO PATHWAY; SELF-RENEWAL; CONTACT INHIBITION; FAMILY PROTEINS; GROUND-STATE; MYC; NAIVE; PROLIFERATION; TROPHECTODERM; ROLES;

D O I：

10.1016/j.devcel.2019.05.024

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The epiblast is a pluripotent cell population first formed in preimplantation embryos, and its quality is important for proper development. Here, we examined the mechanisms of epiblast formation and found that the Hippo pathway transcription factor TEAD and its coactivator YAP regulate expression of pluripotency factors. After specification of the inner cell mass, YAP accumulates in the nuclei and activates TEAD. TEAD activity is required for strong expression of pluripotency factors and is variable in the forming epiblast. Cells showing low TEAD activity are eliminated from the epiblast through cell competition. Pluripotency factor expression and MYC control cell competition downstream of TEAD activity. Cell competition eliminates unspecified cells and is required for proper organization of the epiblast. These results suggest that induction of pluripotency factors by TEAD activity and elimination of unspecified cells via cell competition ensure the production of an epiblast with naive pluripotency.

引用

页码：138 / +

页数：21

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