Cloning and sequence analysis of an intron-containing domain from a peptide synthetase-encoding gene of the entomopathogenic fungus Metarhizium anisopliae

被引：37
作者：
Bailey, AM
Kershaw, MJ
Hunt, BA
Paterson, IC
Charnley, AK
Reynolds, SE
Clarkson, JM
机构：
[1] Microbial Pathogenicity Group, School of Biology and Biochemistry, University of Bath
[2] School of Biology and Biochemistry, University of Bath, Bath BA2 7AY, Claverton Down
来源：
GENE | 1996年 / 173卷 / 02期
基金：
英国生物技术与生命科学研究理事会;
关键词：
destruxin; core sequence; depsipeptide;
D O I：
10.1016/0378-1119(96)00212-0
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A gene encoding a putative peptide synthetase has been cloned and partially sequenced from the filamentous fungus, Metarhizium anisopliae. The deduced amino acid sequence of one entire domain and the following spacer is typical of fungal peptide synthetases, showing good conservation of the six expected core sequences. There are two introns within this region, the first interrupting core 5 (RLDLTDIE) of the domain and the second in a conserved area of the spacer region.
引用
收藏
页码：195 / 197
页数：3
相关论文
共 13 条
[1]   IDENTIFICATION OF PUTATIVE MULTIFUNCTIONAL PEPTIDE SYNTHETASE GENES USING HIGHLY CONSERVED OLIGONUCLEOTIDE SEQUENCES DERIVED FROM KNOWN SYNTHETASES [J].
BORCHERT, S ;
PATIL, SS ;
MARAHIEL, MA .
FEMS MICROBIOLOGY LETTERS, 1992, 92 (02) :175-180
[2]   NUCLEOTIDE-SEQUENCE OF 5' PORTION OF SRFA THAT CONTAINS THE REGION REQUIRED FOR COMPETENCE ESTABLISHMENT IN BACILLUS-SUBTILIS [J].
FUMA, S ;
FUJISHIMA, Y ;
CORBELL, N ;
DSOUZA, C ;
NAKANO, MM ;
ZUBER, P ;
YAMANE, K .
NUCLEIC ACIDS RESEARCH, 1993, 21 (01) :93-97
[3]  
GURR SJ, 1988, GENE STRUCTURE EUKAR, P93
[4]   MOLECULAR CHARACTERIZATION OF THE ENNIATIN SYNTHETASE GENE ENCODING A MULTIFUNCTIONAL ENZYME CATALYZING N-METHYLDEPSIPEPTIDE FORMATION IN FUSARIUM-SCIRPI [J].
HAESE, A ;
SCHUBERT, M ;
HERRMANN, M ;
ZOCHER, R .
MOLECULAR MICROBIOLOGY, 1993, 7 (06) :905-914
[5]   INHIBITORY EFFECTS OF CYCLODEPSIPEPTIDES, DESTRUXINS, FROM THE FUNGUS METARHIZIUM-ANISOPLIAE, ON CELLULAR-IMMUNITY IN INSECTS [J].
HUXHAM, IM ;
LACKIE, AM ;
MCCORKINDALE, NJ .
JOURNAL OF INSECT PHYSIOLOGY, 1989, 35 (02) :97-105
[6]   BIOSYNTHESIS OF DESTRUXINS [J].
JEGOROV, A ;
SEDMERA, P ;
MATHA, V .
PHYTOCHEMISTRY, 1993, 33 (06) :1403-1405
[7]   BIOSYNTHESIS OF PEPTIDE ANTIBIOTICS [J].
KLEINKAUF, H ;
VONDOHREN, H .
ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :259-289
[8]   MULTIDOMAIN ENZYMES INVOLVED IN PEPTIDE-SYNTHESIS [J].
MARAHIEL, MA .
FEBS LETTERS, 1992, 307 (01) :40-43
[9]   CHARACTERIZATION OF THE GENE ENCODING ACETYL-COA SYNTHETASE IN PENICILLIUM-CHRYSOGENUM - CONSERVATION OF INTRON POSITION IN PLECTOMYCETES [J].
MARTINEZBLANCO, H ;
OREJAS, M ;
REGLERO, A ;
LUENGO, JM ;
PENALVA, MA .
GENE, 1993, 130 (02) :265-270
[10]   THE ROLE OF DESTRUXINS IN THE PATHOGENICITY OF 3 STRAINS OF METARHIZIUM-ANISOPLIAE FOR THE TOBACCO HORNWORM MANDUCA-SEXTA [J].
SAMUELS, RI ;
CHARNLEY, AK ;
REYNOLDS, SE .
MYCOPATHOLOGIA, 1988, 104 (01) :51-58
12