Hepatic ischemia-reperfusion promotes liver metastasis of colon cancer

被引：67

作者：

Doi, K ^{[1
]}

Horiuchi, T ^{[1
]}

Uchinami, M ^{[1
]}

Tabo, T ^{[1
]}

Kimura, N ^{[1
]}

Yokomachi, J ^{[1
]}

Yoshida, M ^{[1
]}

Tanaka, K ^{[1
]}

机构：

[1] Fukui Med Univ, Dept Surg 2, Fukui 9101193, Japan

来源：

JOURNAL OF SURGICAL RESEARCH | 2002年 / 105卷 / 02期

关键词：

hepatic ischemia; liver metastasis; colon cancer; E-selectin (ELAM-1);

D O I：

10.1006/jsre.2002.6356

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Background. The effects of hepatic ischemia/reperfusion (I/R) on liver metastasis have not been fully examined. We examined hepatic I/R and liver metastasis of colorectal cancer in a rat model; we also quantitated expression of E-selectin (ELAM-1) mRNA after I/R. Materials and methods. Rats underwent 30 or 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. The number of tumor nodules on the liver surface was determined 3 weeks later. Expression of E-selectin mRNA was determined at 1, 3, and 6 h after ischemia by quantitative RT-PCR. Results. Hepatic I/R promoted liver metastasis of RCN-H4 and induced the expression of E-selectin mRNA in both clamped and unclamped liver lobes. The number of tumor nodules and the expression of E-selectin mRNA after 60 min of ischemia was greater than that after 30 min. Conclusions. Hepatic I/R, especially with a long duration of ischemia, induces expression of E-selectin and promotes liver metastasis of colon cancer in rats. (C) 2002 Elsevier Science (USA).

引用

页码：243 / 247

页数：5

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[1] Sinusoidal endothelium release of hydrogen peroxide enhances very late antigen-4-mediated melanoma cell adherence and tumor cytotoxicity during interleukin-1 promotion of hepatic melanoma metastasis in mice [J].