Hairy Root Cultures of Taxus cuspidata for Enhanced Production of Paclitaxel

被引：20

作者：

Kim, Jung A. ^{[2
]}

Baek, Kwang-Hyun ^{[3
]}

Son, Young Mi ^{[2
]}

Son, Sung Ho ^{[2
,4
]}

Shin, Heungsop ^{[1
]}

机构：

[1] Korea Polytech Univ, Dept Chem Engn & Biotechnol, Shihung 429793, South Korea

[2] VitroSys Inc, Yeongju 750804, South Korea

[3] Yeungnam Univ, Sch Biotechnol, Gyongsan 712749, South Korea

[4] Dongyang Univ, Dept Biochem Engn, Yeongju 750804, South Korea

来源：

JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY | 2009年 / 52卷 / 02期

关键词：

Agrobacterium rhizogenes; bioreactor; hairy root; methyl jasmonate; taxol; Taxus cuspidata Sieb. et Zucc; CELL-SUSPENSION CULTURES; 1ST TOTAL-SYNTHESIS; METHYL JASMONATE; BACCATIN-III; TAXOL; MEDIA; ACCUMULATION; BREVIFOLIA; KINETICS; RING;

D O I：

10.3839/jksabc.2009.027

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Hairy root cultures were established by transforming seedlings of Taxus cuspidata Sieb. Et Zucc (Korean yew) using Agrobacterium rhizogenes. Initially, 107 lines of hairy roots were induced by infection with 3 strains of Agrobacterium (R1000, A4, and 15384), however, only three tines generated from the R1000 strain were stably grown on hormone-free media over 10 months of successive cultures. These lines were designated as RC11104, RC11105 and RC11106. The hairy root tine RC 11106 was selected for further experiments for taxol production based on its growth properties. Upon methyl jasmonate treatment, the RC11106 line accumulated 52.5 mg/L of taxol over 2 weeks of incubation at a 20-L culture scale.

引用

页码：144 / 150

页数：7

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