One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

被引：11
作者：
Zhang, Cuiling [1 ]
Ding, Caiping [1 ]
Zhou, Guohua [2 ]
Xue, Qin [1 ]
Xian, Yuezhong [1 ]
机构：
[1] East China Normal Univ, Dept Chem, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China
[2] Lingnan Normal Univ, Sch Chem & Chem Engn, Zhanjiang 524048, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2017年 / 957卷
基金：
中国国家自然科学基金;
关键词：
Biosensor; FRET; Quantum dots; WS2; Terminal protection of small-molecule; linked DNA; MOLECULE-LINKED DNA; WS2; NANOSHEET; TERMINAL PROTECTION; ONE-POT; SEMICONDUCTOR NANOCRYSTALS; IN-VITRO; PLATFORM; PERFORMANCE; BIOSENSOR; STRATEGY;
D O I：
10.1016/j.aca.2016.12.024
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNAZnS:Mn2+) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PM and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：63 / 69
页数：7
相关论文
共 41 条
  • [1] Size-dependent dissociation pH of thiolate ligands from cadmium chalcogenide nanocrystals
    Aldana, J
    Lavelle, N
    Wang, YJ
    Peng, XG
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (08) : 2496 - 2504
  • [2] Emerging non-traditional Forster resonance energy transfer configurations with semiconductor quantum dots: Investigations and applications
    Algar, W. Russ
    Kim, Hyungki
    Medintz, Igor L.
    Hildebrandt, Niko
    [J]. COORDINATION CHEMISTRY REVIEWS, 2014, 263 : 65 - 85
  • [3] Recent progress in the bioconjugation of quantum dots
    Blanco-Canosa, Juan B.
    Wu, Miao
    Susumu, Kimihiro
    Petryayeva, Eleonora
    Jennings, Travis L.
    Dawson, Philip E.
    Algar, W. Russ
    Medintz, Igor L.
    [J]. COORDINATION CHEMISTRY REVIEWS, 2014, 263 : 101 - 137
  • [4] Semiconductor nanocrystals as fluorescent biological labels
    Bruchez, M
    Moronne, M
    Gin, P
    Weiss, S
    Alivisatos, AP
    [J]. SCIENCE, 1998, 281 (5385) : 2013 - 2016
  • [5] Protein Detection Based on Small Molecule-Linked DNA
    Cao, Ya
    Zhu, Sha
    Yu, Jiacui
    Zhu, Xuejun
    Yin, Yongmei
    Li, Genxi
    [J]. ANALYTICAL CHEMISTRY, 2012, 84 (10) : 4314 - 4320
  • [6] Synthesis and characterization of poly(acrylic acid) stabilized cadmium sulfide quantum dots
    Celebi, Serdar
    Erdamar, A. Koray
    Sennaroglu, Alphan
    Kurt, Adnan
    Acar, Havva Yagei
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2007, 111 (44) : 12668 - 12675
  • [7] Surfactant-Assisted Synthesis and Characterization of Stable Silver Bromide Nanoparticles in Aqueous Media
    Chakraborty, Moumita
    Hsiao, Fang-Wei
    Naskar, Bappaditya
    Chang, Chien-Hsiang
    Panda, Amiya Kumar
    [J]. LANGMUIR, 2012, 28 (18) : 7282 - 7290
  • [8] Quantum dot bioconjugates for ultrasensitive nonisotopic detection
    Chan, WCW
    Nie, SM
    [J]. SCIENCE, 1998, 281 (5385) : 2016 - 2018
  • [9] Dual-color determination of protein via terminal protection of small-molecule-linked DNA and the enzymolysis of exonuclease III
    Chen, Chaohui
    Xiang, Xia
    Liu, Yufei
    Zhou, Guohua
    Ji, Xinghu
    He, Zhike
    [J]. BIOSENSORS & BIOELECTRONICS, 2014, 58 : 205 - 208
  • [10] Bioconjugated quantum dots as fluorescent probes for bioanalytical applications
    Frasco, Manuela F.
    Chaniotakis, Nikos
    [J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 396 (01) : 229 - 240
12345