Mycosynthesis, characterization, anticancer and antibacterial activity of silver nanoparticles from endophytic fungus Talaromyces purpureogenus

被引:90
作者
Hu, Xiaowen [1 ]
Saravanakumar, Kandasamy [1 ]
Jin, Tieyan [2 ]
Wang, Myeong-Hyeon [1 ]
机构
[1] Kangwon Natl Univ, Coll Biomed Sci, Dept Med Biotechnol, Chunchon 200701, South Korea
[2] Yanbian Univ, Coll Agr, Dept Food Sci & Engn, Yanji, Jilin, Peoples R China
来源
INTERNATIONAL JOURNAL OF NANOMEDICINE | 2019年 / 14卷
基金
新加坡国家研究基金会;
关键词
mycosynthesis; silver nanoparticles; Talaromyces purpureogenus; antibacterial; anticancer; cell wound healing; CYTOTOXICITY; ACTIVATION; CELLS; PLANT;
D O I
10.2147/IJN.S200817
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Background: Biogenic silver nanoparticles (AgNPs) have wider range of biomedical applications. The present work synthesized Tp-AgNPs using mycelial extract of endophytic fungus Talaromyces purpureogenus (MEEF), characterized, and analyzed for antibacterial, anti-proliferation and cell wounding healing activities. Methods: The synthesized Tp-AgNPs were characterized by UV-visible spectrophotometer (UV-Vis), field emission transmission electron microscopy (FETEM) with energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), particle size analysis (PSA) and X-ray diffraction (XRD). Further, antibacterial activity was determined by Kirby-Bauer test and anti-proliferation activity was tested in human lung carcinoma A549 by water-soluble tetrazolium and flow cytometer assay. In addition, cell wounding healing activity was determined by scratch assay. Results: UV-Vis results displayed a strong absorption peak from 390 nm to 420 nm, which indicated the successful synthesis of Tp-AgNPs. FETEM-EDS results indicated the round and triangle shaped Tp-AgNPs with the average size of 25 nm in accordance with PSA. FTIR analysis indicated the involvement of various functional molecules from MEEF in the synthesis of Tp-AgNPs. XRD result proved nature of Tp-AgNPs as a high-quality crystal. The Tp-AgNPs significantly inhibited the growth of bacterial pathogens at the minimal inhibitory concentration of 16.12 mu g.mL(-1) for Gram(+), and 13.98 mu g. mL(-1) for Grambacteria. Further, Tp-AgNPs (2 mu g.mL(-1)) showed a strong anti-proliferation effect in A549. Interestingly, Tp-AgNPs was not cytotoxic to normal NIH3T3 cells. In addition, the NPs exhibited a strong cell wounding healing activity. Conclusion: This work biosynthesized AgNPs with strong antibacterial, anticancer and cell wound healing properties using endophytic fungus T. purpureogenus.
引用
收藏
页码:3427 / 3438
页数:12
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