A simple entrapment of glucoamylase into LentiKats® as an efficient catalyst for maltodextrin hydrolysis

被引：48
作者：
Rebros, Martin [1 ]
Rosenberg, Michal [1 ]
Mlichova, Zuzana [1 ]
Kristofikova, L'udmila [1 ]
Paluch, Miroslav [1 ]
机构：
[1] Slovak Univ Technol Bratislava, Dept Biotechnol, Fac Chem & Food Technol, Bratislava 81237, Slovakia
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 39卷 / 04期
关键词：
glucoamylase; immobilization; LentiKats((R)); poly(vinylalcohol); PVA gel;
D O I：
10.1016/j.enzmictec.2006.01.001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The glucoamylase from Aspergillus niger was immobilized into a poly(vinylalcohol) hydrogel lens-shaped capsules LentiKats (R). Immobilization broadened the pH optimum of the enzyme. The K-M of glucoamylase increased after immobilization (approximately 1.5 times on maltose), which reflected the decrease in affinity of enzyme for its substrate. Immobilized enzyme retained excellent long-term operational stability for both, batch and continuous mode of hydrolysis. Specific enzymatic activity of the immobilized enzyme (0.67 g g(-1) h(-1)) was constant for 63 days of continuous operation at 45 degrees C. (c) 2006 Elsevier Inc. All rights reserved.
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页码：800 / 804
页数：5
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