Urinary MicroRNA-30c-5p and MicroRNA-192-5p as potential biomarkers of ischemia-reperfusion-induced kidney injury

被引:65
作者
Zou, Yan-Fang [1 ]
Wen, Dan [1 ]
Zhao, Qian [2 ]
Shen, Ping-Yan [1 ]
Shi, Hao [1 ]
Zhao, Qiang [3 ]
Chen, Yong-Xi [1 ]
Zhang, Wen [1 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Nephrol, Shanghai 200025, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Key Lab Cell Differentiat & Apoptosis, Natl Minist Educ, Shanghai 200025, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Cardiovasc Surg, Shanghai 200025, Peoples R China
基金
中国国家自然科学基金;
关键词
Ischemia/reperfusion; kidney injury; microRNAs; biomarkers; CIRCULATING MICRORNAS; AKI; BIOGENESIS; EXPRESSION; CANCER; PERFORMANCE; METASTASIS; DISEASES; PROFILE; RISK;
D O I
10.1177/1535370216685005
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Early detection of acute kidney injury is difficult due to lack of known biomarkers; previous studies have tried to identify new biomarkers for detecting acute kidney injury at an early stage. MicroRNA, a 21-23 nucleotide noncoding RNA molecule, has emerged as a desirable marker in the diagnosis and prognosis of various diseases. This study aims to identify the expression profile of microRNA in ischemia-reperfusion-induced kidney injury and determine the possibility of using the candidate microRNA as biomarker for the detection of I/R-induced kidney injury. Based on the established rat model of I/R-induced kidney injury, a microarray analysis of rat urine was performed at the beginning of operation (0 h) as well as 72 h post operation. To validate the results, urine samples from 71 patients who underwent cardiac surgery were collected, after which urinalysis was conducted to determine the microRNA concentration. An alternative expression profile of microRNAs was detected in rat urine. The quantitative validation of microRNA showed that the expression of miR-30c-5p, miR-192-5p, and miR-378a-3p was elevated significantly in urine post operation, which was consistent with those of the microarray analysis and earlier than kidney injury molecule-1 (KIM-1). In patients with acute kidney injury, increased levels of miR-30c-5p and miR-192-5p were also detected 2 h post operation, and miR-30c-5p showed preferable diagnostic value compared with protein-based biomarkers. In conclusion, an aberrant expression profile of microRNA was detected in rat urine based on the established ischemia-reperfusion animal model. Both miR-30c-5p and miR-192-5p served as important potential diagnostic markers for I/R-induced kidney injury.
引用
收藏
页码:657 / 667
页数:11
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