Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta

被引：74
|
作者：
Zhang Haibo [1 ]
Zhang Yinglong [1 ]
Huang Feng [1 ,2 ]
Gao Peiji [1 ]
Chen Jiachuan [2 ]
机构：
[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China
[2] Shandong Inst Light Ind, Shandong Key Lab Pulp & Paper Engn, Jinan 250353, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 06期
基金：
美国国家科学基金会;
关键词：
Fungi; Laccase; Mediator; Purification; Thermostability; FUNGAL LACCASES; YELLOW LACCASE; VERSICOLOR;
D O I：
10.1007/s10529-009-9945-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A laccase was purified from Trametes hirsuta. This laccase was classified as a "white" or "yellow" laccase. pH 2.4 was optimal for the oxidation of ABTS and pH 2.5 for DMP. DMP oxidation was optimal at 85A degrees C. The half-life of this laccase was 70 min at 75A degrees C, and 5 h at 65A degrees C. Non-phenolic dyes, such as Methyl Red, were oxidized by purified laccase without mediators. The enzyme was not inhibited by Cu2+, Mn2+, or EDTA. These are atypical laccase characteristics that make it a good candidate for theoretical and applied research.
引用
收藏
页码：837 / 843
页数：7
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