Color recovery in berries of grape (Vitis vinifera L.) 'Benitaka', a bud sport of 'Italia', is caused by a novel allele at the VvmybA1 locus

被引:89
作者
Azuma, Akifumi [1 ]
Kobayashi, Shozo [1 ]
Goto-Yamamoto, Nami [2 ]
Shiraishi, Mikio [3 ]
Mitani, Nobuhito [1 ]
Yakushiji, Hiroshi [1 ]
Koshita, Yoshiko [1 ]
机构
[1] Natl Inst Fruit Tree Sci, Grape & Persimmon Res Stn, Hiroshima 7392494, Japan
[2] Natl Res Inst Brewing, Hiroshima 7392494, Japan
[3] Fukuoka Agr Res Ctr, Fukuoka 8188519, Japan
关键词
Anthocyanin; myb; Grape skin color; Bud mutation; MYB-RELATED GENES; ANTHOCYANIN BIOSYNTHESIS; EXPRESSION; SKIN; MUTATION; DELETION; REPEATS; DROUGHT; PATHWAY; RED;
D O I
10.1016/j.plantsci.2008.12.015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Color mutations in grape berry skin are relatively frequent events, and can be easily seen in the vineyard. Both light-red-skinned 'Ruby Okuyama' and more intense and uniform rosy-skinned 'Benitaka' (Vitis vinifera L.) are bud sports of white-skinned 'Italia'. Previously, we reported that 'Ruby Okuyama' was caused by the recovery of VvmybA1 expression, which may have occurred as a result of intra-LTR (long terminal repeat) recombination within a retrotransposon, Gret1. However, the molecular basis of the color recovery in 'Benitaka' has not been elucidated so far. Here, we found that the VvmybA1 locus of 'Benitaka' is heterozygous for the VvmybA1a allele (non-functional) and a novel VvmybA1(BEN) allele, and that VvmybA1(BEN) restored VvmybA1 transcripts. We hypothesized that VvmybA1(BEN) allele was caused by homologous recombination between VvmybA1a and VvmybA3. In addition, the content and composition of anthocyanins in berry skins differed greatly between 'Ruby Okuyama' and 'Benitaka'. The levels of expression of the genes for flavonoid 3',5'-hydroxylase (F3'5'H), O-methyltransferase (OMT), and glutathione-S-transferase (GST) were associated with differences in the anthocyanin content and composition between the two cultivars. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:470 / 478
页数:9
相关论文
共 39 条
[1]  
Azuma A, 2007, VITIS, V46, P154
[2]   Genomic and genetic analysis of Myb-related genes that regulate anthocyanin biosynthesis in grape berry skin [J].
Azuma, Akifumi ;
Kobayashi, Shozo ;
Mitani, Nobuhito ;
Shiraishi, Mikio ;
Yamada, Masahiko ;
Ueno, Toshihito ;
Kono, Atsushi ;
Yakushiji, Hiroshi ;
Koshita, Yoshiko .
THEORETICAL AND APPLIED GENETICS, 2008, 117 (06) :1009-1019
[3]   Identification of the flavonoid hydroxylases from grapevine and their regulation during fruit development [J].
Bogs, J ;
Ebadi, A ;
McDavid, D ;
Robinson, SP .
PLANT PHYSIOLOGY, 2006, 140 (01) :279-291
[4]  
Boss P. K., 1996, Australian Journal of Grape and Wine Research, V2, P163, DOI 10.1111/j.1755-0238.1996.tb00104.x
[5]   Expression of anthocyanin biosynthesis pathway genes in red and white grapes [J].
Boss, PK ;
Davies, C ;
Robinson, SP .
PLANT MOLECULAR BIOLOGY, 1996, 32 (03) :565-569
[6]   Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L) [J].
Bowers, JE ;
Dangl, GS ;
Vignani, R ;
Meredith, CP .
GENOME, 1996, 39 (04) :628-633
[7]  
Bray EA., 1994, Stress Induced Gene Expression in Plants eds, P1
[8]   Regulation of abscisic acid-induced transcription [J].
Busk, PK ;
Pagès, M .
PLANT MOLECULAR BIOLOGY, 1998, 37 (03) :425-435
[9]   Colour variation in red grapevines (Vitis vinifera L.):: genomic organisation, expression of flavonoid 3′-hydroxylase, flavonoid 3′,5′-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyanins in berry skin [J].
Castellarin, SD ;
Di Gaspero, G ;
Marconi, R ;
Nonis, A ;
Peterlunger, E ;
Paillard, S ;
Adam-Blondon, AF ;
Testolin, R .
BMC GENOMICS, 2006, 7 (1)
[10]   Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines [J].
Castellarin, Simone D. ;
Di Gaspero, Gabriele .
BMC PLANT BIOLOGY, 2007, 7