Hybrid approaches: applying computational methods in cryo-electron microscopy

被引：28

作者：

Lindert, Steffen ^{[1
,3
]}

Stewart, Phoebe L. ^{[2
,3
]}

Meiler, Jens ^{[1
,3
]}

机构：

[1] Vanderbilt Univ, Dept Chem, Nashville, TN 37212 USA

[2] Vanderbilt Univ, Med Ctr, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA

[3] Vanderbilt Univ, Struct Biol Ctr, Nashville, TN 37232 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2009年 / 19卷 / 02期

基金：

美国国家科学基金会;

关键词：

STRUCTURAL-INFORMATICS APPROACH; HIGH-RESOLUTION STRUCTURE; CRYO-EM; ELECTRON CRYOMICROSCOPY; STRUCTURE PREDICTION; PROTEIN SECONDARY; ATOMIC STRUCTURES; BETA-SHEETS; REFINEMENT; MAPS;

D O I：

10.1016/j.sbi.2009.02.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in cryo-electron microscopy have led to an increasing number of high (3-5 angstrom) to medium (5-10 angstrom) resolution cryoEM density maps. These density maps contain valuable information about the protein structure but frequently require computational algorithms to aid their structural interpretation. It is these hybrid approaches between cryoEM and computational protein structure prediction algorithms that will shape protein structure elucidation from density maps.

引用

页码：218 / 225

页数：8

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