Novel Combi-lipase Systems for Fatty Acid Ethyl Esters Production

被引:29
作者
Toro, Esteban C. [1 ]
Rodriguez, Diego F. [1 ,2 ]
Morales, Nelson [1 ]
Garcia, Lina M. [1 ]
Godoy, Cesar A. [1 ]
机构
[1] Univ Valle, Grp Invest Ingn Proc Agroalimentarios & Biotecnol, Dept Quim LIBB, Cali 76001, Colombia
[2] Pontificia Univ Catolica Chile, Fac Quim & Farm, Vicuna Mackenna 4860, Santiago 7820436, Chile
来源
CATALYSTS | 2019年 / 9卷 / 06期
关键词
immobilization; Combi-lipases; support; biodiesel; lipases; transesterification; BIODIESEL PRODUCTION; CATALYZED TRANSESTERIFICATION; IMMOBILIZED LIPASE; HYDROPHOBIC SUPPORTS; KINETIC-MODEL; SOYBEAN OIL; COIMMOBILIZATION; ESTERIFICATION; OPTIMIZATION; HYDROLYSIS;
D O I
10.3390/catal9060546
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Most Combi-lipases (CL) are based on mixtures of different lipases immobilized on different supports. The increased CL efficiency has been attributed solely to the complementary selectivity of lipases. However, the role of the immobilization support in CL or in co-immobilized systems (coCL) and the application of kinetic models to account CL composition effects, have not been assessed. In this work, commercial lipases from Thermomyces lunuginosus (TLL), Candida antarctica (CALB) and Rhizomocur miehei (RML) and supports as Lewatit((R))VPOC1600 (LW) and Purolite((R))ECR1604 (PU), were combined to produce new CL systems for the production of fatty acid ethyl esters (EE) which are the main component of ethylic biodiesel: Co-immobilization slightly altered palm olein EE yields with regard to that of equivalent CL systems, e.g., the best coCL of TLL and CALB in LW (89.5%) and the respective CL (81.8%). The support did affect CL behavior: (i) The best coCL of TLL and RML on LW produced 80.0% EE while on PU 76.4%; (ii) CL based on mixtures of the same enzyme, but immobilized on different supports (semiCL) show complementarity: The best TLL semiCL produced 86.1% EE while its constituents (LW) and (PU) produced individually 78.2 and 70.3%, respectively. The proposed model accounts adequately the EE production properties for CL systems based on TLL, CALB and LW. This work expands the tools to obtain new CL systems for EE production.
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页数:16
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