Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures

被引：56
|
作者：
Pasteran, Fernando [1 ]
Tijet, Nathalie [2 ]
Melano, Roberto G. [2 ,3 ,4 ]
Corso, Alejandra [1 ]
机构：
[1] Minist Salud Nac, Serv Antimicrobianos, Lab Nacl Reg Referencia Antimicrobianos, INEI,ANLIS Dr Carlos G Malbran, Buenos Aires, DF, Argentina
[2] PHOL, Toronto, ON, Canada
[3] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada
[4] Mt Sinai Hosp, Dept Microbiol, Toronto, ON M5G 1X5, Canada
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 12期
关键词：
PROVIDENCIA-RETTGERI STRAINS; GRAM-NEGATIVE BACILLI; RAPID DETECTION;
D O I：
10.1128/JCM.02032-15
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the CLSI tests). The specificities were comparable (100%), but the CNPtdirect was more sensitive (98% versus 84%). The CNPt-direct was easier to perform due to the direct use of colonies and offered a more robust detection of carbapenemase producers.
引用
收藏
页码：3908 / 3911
页数：4
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