Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures

被引:56
|
作者
Pasteran, Fernando [1 ]
Tijet, Nathalie [2 ]
Melano, Roberto G. [2 ,3 ,4 ]
Corso, Alejandra [1 ]
机构
[1] Minist Salud Nac, Serv Antimicrobianos, Lab Nacl Reg Referencia Antimicrobianos, INEI,ANLIS Dr Carlos G Malbran, Buenos Aires, DF, Argentina
[2] PHOL, Toronto, ON, Canada
[3] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada
[4] Mt Sinai Hosp, Dept Microbiol, Toronto, ON M5G 1X5, Canada
关键词
PROVIDENCIA-RETTGERI STRAINS; GRAM-NEGATIVE BACILLI; RAPID DETECTION;
D O I
10.1128/JCM.02032-15
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the CLSI tests). The specificities were comparable (100%), but the CNPtdirect was more sensitive (98% versus 84%). The CNPt-direct was easier to perform due to the direct use of colonies and offered a more robust detection of carbapenemase producers.
引用
收藏
页码:3908 / 3911
页数:4
相关论文
共 20 条
  • [1] Evaluation of the Carba NP Test for Carbapenemase Detection
    Osterblad, Monica
    Hakanen, Antti J.
    Jalava, Jari
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2014, 58 (12) : 7553 - 7556
  • [2] Detection of carbapenemase-producing gram-negative bacteria using a simplified Carba NP test
    Yan, Qun
    Liu, Qingxia
    Li, Yanming
    Li, Hongling
    Liang, Xianghui
    Zou, Mingxiang
    Liu, Wen-en
    JOURNAL OF MICROBIOLOGICAL METHODS, 2016, 123 : 1 - 3
  • [3] Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in Bacteroides Species
    Akyar, Isin
    Ayas, Meltem
    Karatuna, Onur
    Besli, Yesim
    POLISH JOURNAL OF MICROBIOLOGY, 2018, 67 (01) : 97 - 101
  • [4] Carba NP test for the detection of carbapenemase-producing Pseudomonas aeruginosa
    Bouslah, Z.
    MEDECINE ET MALADIES INFECTIEUSES, 2020, 50 (06): : 466 - 479
  • [5] Evaluation of the RAPIDEC® CARBA NP, the Rapid CARB Screen® and the Carba NP test for biochemical detection of carbapenemase-producing Enterobacteriaceae
    Dortet, Laurent
    Agathine, Aurelie
    Naas, Thierry
    Cuzon, Gaelle
    Poirel, Laurent
    Nordmann, Patrice
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2015, 70 (11) : 3014 - 3022
  • [6] Cilastatin does not affect Carba NP test performance for detection of carbapenemase production in Enterobacteriaceae
    Hartl, Rainer
    Widhalm, Sarah
    Kerschner, Heidrun
    Apfalter, Petra
    Gattringer, Rainer
    WIENER KLINISCHE WOCHENSCHRIFT, 2017, 129 (1-2) : 29 - 32
  • [7] On-chip Carba NP test for accurate and high throughput detection of carbapenemase-producing Enterobacteriaceae
    Wasey, Abdul
    Yang, Jianhua
    Sun, Dan
    He, Yuan
    Zhang, Ce
    TALANTA, 2020, 210 (210)
  • [8] Laboratory detection of intestinal carriage of carbapenemase-producing Enterobacteriaceae - A comparison of algorithms using the Carba NP test
    Knox, James
    Gregory, Claire
    Prendergast, Louise
    Perera, Chandrika
    Robson, Jennifer
    Waring, Lynette
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2017, 87 (01) : 17 - 21
  • [9] Phenotypic Detection of Carbapenemase Production in Carbapenem-Resistant Enterobacteriaceae by Modified Hodge Test and Modified Strip Carba NP Test
    Patidar, Nisha
    Vyas, Nitya
    Sharma, Shanoo
    Sharma, Babita
    JOURNAL OF LABORATORY PHYSICIANS, 2021, 13 (01) : 14 - 21
  • [10] A Rapid Carbapenemase Genes Detection Method with Xpert Carba-R from Positive Blood Cultures Compared with NG-Test Carba 5 and Sequencing
    Xu, Yuqiao
    Song, Weijuan
    Huang, Peijun
    Mei, Yaning
    Zhang, Yan
    Xu, Ting
    INFECTION AND DRUG RESISTANCE, 2022, 15 : 7719 - 7725