CRISPR-Cas9; an efficient tool for precise plant genome editing

被引：7

作者：

Islam, Waqar ^{[1
,2
,3
]}

机构：

[1] Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou 350002, Fujian, Peoples R China

[2] State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Fujian, Peoples R China

[3] Govt Punjab, Dept Agr, Lahore, Pakistan

来源：

MOLECULAR AND CELLULAR PROBES | 2018年 / 39卷

关键词：

Genetic mutation; Genetic engineering; Homologous recombination; DNA; Transcription; CHROMOSOMAL DELETIONS; GENE-EXPRESSION; RNA; CRISPR/CAS9; ARABIDOPSIS; CAS9; RICE; SEQUENCE; SYSTEM; TOMATO;

D O I：

10.1016/j.mcp.2018.03.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Efficient plant genome editing is dependent upon induction of double stranded DNA breaks (DSBs) through site specified nucleases. These DSBs initiate the process of DNA repair which can either base upon homologous recombination (HR) or non-homologous end jointing (NHEJ). Recently, CRISPR-Cas9 mechanism got highlighted as revolutionizing genetic tool due to its simpler frame work along with the broad range of adaptability and applications. So, in this review, I have tried to sum up the application of this biotechnological tool in plant genome editing. Furthermore, I have tried to explain successful adaptation of CRISPR in various plant species where it is used for the successful generation of stable mutations in a steadily growing number of species through NHEJ. The review also sheds light upon other biotechnological approaches relying upon single DNA lesion induction such as genomic deletion or pair wise nickases for evasion of offsite effects.

引用

页码：47 / 52

页数：6

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