Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides

被引：33

作者：

Grey, Carl ^{[1
,2
]}

Edebrink, Per ^{[2
,3
]}

Krook, Maria ^{[2
,3
]}

Jacobsson, Sven P. ^{[4
]}

机构：

[1] Lund Univ, Dept Biotechnol, SE-22100 Lund, Sweden

[2] AstraZeneca R&D, BioPR&D, SE-15185 Sodertalje, Sweden

[3] Recipharm Biol, SE-15257 Sodertalje, Sweden

[4] Stockholm Univ, Dept Analyt Chem, SE-10691 Stockholm, Sweden

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 20-21期

关键词：

HPAEC-PAD; MALDI-TOF MS; Oligosaccharides; N-glycosylation; Glycoprotein; Monoclonal antibodies; PULSED AMPEROMETRIC DETECTION; FLIGHT MASS-SPECTROMETRY; CAPILLARY-ELECTROPHORESIS; ALPHA(1)-ACID GLYCOPROTEIN; MONOCLONAL-ANTIBODIES; CARBOHYDRATE ANALYSIS; PH; SEPARATION; GLYCOPEPTIDES; IONIZATION;

D O I：

10.1016/j.jchromb.2009.05.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1827 / 1832

页数：6

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