The vaccine efficacy of recombinant duck enteritis virus expressing secreted E with or without PrM proteins of duck tembusu virus

被引：52

作者：

Chen, Pucheng ^{[1
]}

Liu, Jinxiong ^{[1
]}

Jiang, Yongping ^{[1
]}

Zhao, Yuhui ^{[1
]}

Li, Qimeng ^{[1
]}

Wu, Li ^{[1
]}

He, Xijun ^{[1
]}

Chen, Hualan ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

来源：

VACCINE | 2014年 / 32卷 / 41期

关键词：

Recombinant DEVs; Duck tembusu virus; Vector vaccine; WEST-NILE-VIRUS; COMPLETE GENOME SEQUENCE; ENVELOPE PROTEIN; NEUTRALIZING ANTIBODY; FLAVIVIRUS; ENCEPHALITIS; MICE; DNA; CHINA; IMMUNIZATION;

D O I：

10.1016/j.vaccine.2014.07.082

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A newly emerged tembusu virus that causes egg-drop has been affecting ducks in China since 2010. Currently, no vaccine is available for this disease. A live attenuated duck enteritis virus (DEV; a herpesvirus) vaccine has been used routinely to control lethal DEV in ducks since the 1960s. Here, we constructed two recombinant DEVs by transfecting overlapping fosmid DNAs. One virus, rDEV-TE, expresses the truncated form of the envelope glycoprotein (TE) of duck tembusu virus (DTMUV), and the other virus, rDEV-PrM/TE, expresses both the TE and pre-membrane proteins (PrM). Animal study demonstrated that both recombinant viruses induced measurable anti-DTMUV neutralizing antibodies in ducks. After two doses of recombinant virus, rDEV-PrM/TE completely protected ducks from DTMUV challenge, whereas rDEV-TE only conferred partial protection. These results demonstrate that recombinant DEV expressing the TE and pre-membrane proteins is protective and can serve as a potential candidate vaccine to prevent DTMUV infection in ducks. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：5271 / 5277

页数：7

共 32 条

[1]

Aberle JH, 1999, J IMMUNOL, V163, P6756

[2] SYNTHESIS AND SECRETION OF RECOMBINANT TICK-BORNE ENCEPHALITIS-VIRUS PROTEIN-E IN SOLUBLE AND PARTICULATE FORM [J].