Endothelin-1 secretion from cultured vascular endothelial cells of DOCA-salt hypertensive rats

被引：0

作者：

Takada, K ^{[1
]}

Matsumura, Y ^{[1
]}

Dohmen, S ^{[1
]}

Mitsutomi, N ^{[1
]}

Takaoka, M ^{[1
]}

Morimoto, S ^{[1
]}

机构：

[1] OSAKA UNIV PHARMACEUT SCI,DEPT PHARMACOL,TAKATSUKI 56911,OSAKA,JAPAN

来源：

LIFE SCIENCES | 1996年 / 59卷 / 09期

关键词：

endothelin-1; endothelial cells; DOCA-salt hypertension;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The profile of endothelin-1 (ET-1) release from cultured vascular endothelial cells (ECs) obtained from deoxycorticosterone acetate (DOCA)-salt hypertensive rats, was examined and compared with that from normotensive sham rats. ET-1 release from ECs was increased in a time-dependent manner, and the level of DOCA-salt hypertensive rats was higher than that of sham rats. Incubation of ECs with transforming growth factor (TGF)-beta 1 or thrombin resulted in a significant increase in the ET-1 release, while FK409,a novel nitric oxide donor, produced a dose-dependent decrease in the release. In the case of ECs from DOCA-salt hypertensive rats, the potencies of TGF-beta 1 or thrombin-induced action was much less than that seen with sham rats, while the difference of reactivity to FK409 was not observed between ECs of DOCA-salt rats and sham rats. Thus, ET-1 production in ECs appears to be up-regulated in DOCA-salt hypertensive rats. In addition, there seems to be an abnormalities in the signaling pathway via TGF-beta 1- or thrombin-induced enhancement of ET-1 production in ECs of DOCA-salt hypertensive rats.

引用

页码：PL111 / PL116

页数：6

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