Ljungan Virus Present in Intrauterine Fetal Death Diagnosed by Both Immunohistochemistry and PCR

被引：9

作者：

Samsioe, Annika ^{[1
]}

Papadogiannakis, Nikos ^{[2
]}

Hultman, Thomas ^{[3
]}

Sjoholm, Ake ^{[1
]}

Klitz, William ^{[4
,5
]}

Niklasson, Bo ^{[3
,6
]}

机构：

[1] Karolinska Inst, Soder Sjukhuset, Dept Clin Sci & Educ, Sect Internal Med, SE-11883 Stockholm, Sweden

[2] Karolinska Univ Hosp, Dept Pathol, Stockholm, Sweden

[3] Apodemus AB, Stockholm, Sweden

[4] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA

[5] Inst Publ Hlth, Oakland, CA USA

[6] Uppsala Univ, Dept Med Cell Biol, Uppsala, Sweden

来源：

BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY | 2009年 / 85卷 / 03期

关键词：

IUFD; Ljungan virus; PCR; Immunohistochemistry; Picorna virus; ANTIGEN RETRIEVAL; VOLES;

D O I：

10.1002/bdra.20554

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

OBJECTIVES: Following up on prior evidence from animal and human studies of Ljungan virus (LV) in intrauterine fetal death (IUFD), we examine additional cases of IUFD using two standard assays of viral detection: immunohistochemistry (IHC) and real time RT-PCR. MATERIALS AND METHODS: Frozen and formalin-fixed specimens from IUFD cases were tested for the presence of LV using real time RT-PCR and IHC, respectively. Formalin-fixed organs from terminated pregnancies diagnosed as trisomy 21 were used as controls in the IHC assay. RESULTS: Presence of LV was demonstrated in all five IUFD cases by IHC and further confirmed in. three of these cases by real time RT-PCR. Only one of 18 trisomy 21 controls was LV positive by IHC. CONCLUSION: The presence of LV in IUFD patients has been confirmed by two different assays. Birth Defects Research (Part A) 85:227-229, 2009. (C) 2009 Wiley-Liss, Inc.

引用

页码：227 / 229

页数：3

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