Spinal laminae I-II neurons in rat recorded in vivo in whole cell, tight seal configuration: Properties and opioid responses

被引:73
作者
Light, AR
Willcockson, HH
机构
[1] Univ N Carolina, Dept Cell & Mol Physiol, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Curriculum Neurobiol, Chapel Hill, NC 27599 USA
关键词
D O I
10.1152/jn.1999.82.6.3316
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Using the in vivo whole cell recording procedure described previously, we recorded 73 neurons in laminae I and II: in the lumbar spinal cord of the rat. Input impedances averaged 332 M Omega, which indicated that prior sharp electrode recordings contained a significant current shunt. Characterization of the adequate stimuli from the excitatory hindlimb receptive held indicated that 39 of 73 neurons were nociceptive, 6 were innocuous cooling cells, 20 responded maximally to brush, and 8 cells were not excited by stimulation of the skin of the hindlimb. The locations of 15 neurons were marked with biocytin. Nociceptive neurons were mostly found in lamina I and outer II, cooling cells in lamina I, and innocuous mechanoreceptive cells were mostly found in inner II or in the overlying white matter. The mu-opioid agonist [D-Ala(2), N-Me-Phe(4), Gly(5)-ol]-Enkephalin (DAMGO) hyperpolarized 7 of 19 tested neurons with a conductance increase. This hyperpolarization was reversed by naloxone in the neurons in which it was applied. DAMGO also decreased the frequency of spontaneous PSPs in 13 neurons, 7 of which were also hyperpolarized by DAMGO. Five of the seven hyperpolarized neurons were nociceptive, responding to both heat and mechanically noxious stimuli, whereas two responded to slow, innocuous brush. These results indicate that whole cell, tight seal recordings sample a similar population of lamina I and II neurons in the rat as those found with sharp electrode recordings in cat and monkey. They further indicate that DAMGO hyperpolarizes a subset of the nociceptive neurons that have input from both heat and mechanical nociceptors and that presynaptic DAMGO effects can be observed in nociceptive neurons that are not hyperpolarized by DAMGO.
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页码:3316 / 3326
页数:11
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共 57 条
[11]   Nociceptive and thermoreceptive lamina I neurons are anatomically distinct [J].
Han, ZS ;
Zhang, ET ;
Craig, AD .
NATURE NEUROSCIENCE, 1998, 1 (03) :218-225
[12]   DISTINCT ANTINOCICEPTIVE ACTIONS MEDIATED BY DIFFERENT OPIOID RECEPTORS IN THE REGION OF LAMINA I AND LAMINAE III-V OF THE DORSAL HORN OF THE RAT [J].
HOPE, PJ ;
FLEETWOODWALKER, SM ;
MITCHELL, R .
BRITISH JOURNAL OF PHARMACOLOGY, 1990, 101 (02) :477-483
[13]   PRESYNAPTIC INHIBITORY-ACTION OF ENKEPHALIN ON EXCITATORY TRANSMISSION IN SUPERFICIAL DORSAL HORN OF RAT SPINAL-CORD [J].
HORI, Y ;
ENDO, K ;
TAKAHASHI, T .
JOURNAL OF PHYSIOLOGY-LONDON, 1992, 450 :673-685
[14]   A VERSATILE MEANS OF INTRACELLULAR LABELING - INJECTION OF BIOCYTIN AND ITS DETECTION WITH AVIDIN CONJUGATES [J].
HORIKAWA, K ;
ARMSTRONG, WE .
JOURNAL OF NEUROSCIENCE METHODS, 1988, 25 (01) :1-11
[15]   EXPANSION OF RECEPTIVE-FIELDS OF SPINAL LAMINA-I PROJECTION NEURONS IN RATS WITH UNILATERAL ADJUVANT-INDUCED INFLAMMATION - THE CONTRIBUTION OF DORSAL HORN MECHANISMS [J].
HYLDEN, JLK ;
NAHIN, RL ;
TRAUB, RJ ;
DUBNER, R .
PAIN, 1989, 37 (02) :229-243
[16]   MEMBRANE-PROPERTIES OF NOCICEPTIVE NEURONS IN LAMINA-II OF LUMBAR SPINAL-CORD IN THE CAT [J].
IGGO, A ;
MOLONY, V ;
STEEDMAN, WM .
JOURNAL OF PHYSIOLOGY-LONDON, 1988, 400 :367-380
[18]   REPETITIVE STIMULATION-INDUCED POTENTIATION OF EXCITATORY TRANSMISSION IN THE RAT DORSAL HORN - AN IN-VITRO STUDY [J].
JEFTINIJA, S ;
URBAN, L .
JOURNAL OF NEUROPHYSIOLOGY, 1994, 71 (01) :216-228
[19]   Active properties of neuronal dendrites [J].
Johnston, D ;
Magee, JC ;
Colbert, CM ;
Christie, BR .
ANNUAL REVIEW OF NEUROSCIENCE, 1996, 19 :165-186
[20]   EFFECTS OF IONTOPHORESED OPIOIDS ON PHYSIOLOGICALLY CHARACTERIZED LAMINAE-I AND LAMINAE-II DORSAL HORN NEURONS IN THE CAT SPINAL-CORD [J].
JONES, SL ;
SEDIVEC, MJ ;
LIGHT, AR .
BRAIN RESEARCH, 1990, 532 (1-2) :160-174