Identification of Human Pituitary Growth Hormone Variants by Mass Spectrometry

被引：29

作者：

Kohler, Maxie ^{[1
]}

Thomas, Andreas ^{[1
]}

Pueschel, Klaus ^{[2
]}

Schaenzer, Wilhelm ^{[1
]}

Thevis, Mario ^{[1
]}

机构：

[1] German Sport Univ Cologne, Ctr Prevent Doping Res, Inst Biochem, D-50933 Cologne, Germany

[2] Univ Hosp Hamburg Eppendorf, Dept Legal Med, Hamburg, Germany

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 02期

关键词：

2D-PAGE; orbitrap mass spectrometry; glycosylation; fragment; sport; DIMERIC FORMS; RADIOIMMUNOASSAY; GLYCOPROTEINS; PROTEINS; EXTRACTS; ISOFORMS; HEALTHY; WOMEN; HGH;

D O I：

10.1021/pr800945b

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The heterogeneity of human endogenous growth hormone (GH) is used in doping control analysis to distinguish it from the homogeneous recombinant analogue in plasma samples. Pituitary GH variants were characterized by gel electrophoresis and mass spectrometry. Besides 22 and 20 We isoforms, fragments of 9 and 12 kDa were identified and a glycosylated 23 kDa GH variant was elucidated to bear a HexHexNac*2 NeuAc modification presumably located at Thr 60.

引用

页码：1071 / 1076

页数：6

共 29 条

[1] GROWTH-HORMONE HETEROGENEITY - GENES, ISOHORMONES, VARIANTS, AND BINDING-PROTEINS [J].

BAUMANN, G .

ENDOCRINE REVIEWS, 1991, 12 (04) :424-449

[2] Phosphoproteomic analysis of the human pituitary [J].

Beranova-Giorgianni S. ;

Zhao Y. ;

Desiderio D.M. ;

Giorgianni F. .

Pituitary, 2006, 9 (2) :109-120

[3] Short-term administration of supraphysiological recombinant human growth hormone (GH) does not increase maximum endurance exercise capacity in healthy, active young men and women with normal GH-insulin-like growth factor I axes [J].

Berggren, A ;

Ehrnborg, C ;

Thord, R ;

Ellegård, L ;

Bengtsson, BÅ ;

Caidahl, K .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2005, 90 (06) :3268-3273

[4] CHARACTERIZATION OF DIMERIC FORMS OF HUMAN PITUITARY GROWTH-HORMONE BY BIOASSAY, RADIORECEPTOR ASSAY, AND RADIOIMMUNOASSAY [J].

BROSTEDT, P ;

LUTHMAN, M ;

WIDE, L ;

WERNER, S ;

ROOS, P .

ACTA ENDOCRINOLOGICA, 1990, 122 (02) :241-248

[5] ISOLATION OF DIMERIC FORMS OF HUMAN PITUITARY GROWTH-HORMONE [J].

BROSTEDT, P ;

ROOS, P .

PREPARATIVE BIOCHEMISTRY, 1989, 19 (03) :217-229

[6]

*CTR BIOL SEQ AN, NETOGLYC 3 1 SERV HO

[7] Growth hormone isoforms, segments/fragments: Does a link exist with multifunctionality? [J].

De Palo, EF ;

Gatti, R ;

Antonelli, G ;

Spinella, P .

CLINICA CHIMICA ACTA, 2006, 364 (1-2) :77-81

[8] A 12-KILODALTON N-GLYCOSYLATED GROWTH HORMONE-RELATED PEPTIDE IS PRESENT IN HUMAN PITUITARY EXTRACTS [J].

DIAZ, MJ ;

DOMINGUEZ, F ;

HARO, LS ;

LING, N ;

DEVESA, J .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1993, 77 (01) :134-138

[9] Growth hormone deficiency in the adult [J].

Doga M. ;

Bonadonna S. ;

Gola M. ;

Mazziotti G. ;

Giustina A. .

Pituitary, 2006, 9 (4) :305-311

[10] N-glycosylated variants of growth hormone in human pituitary extracts [J].

García-Barros, M ;

Costoya, JA ;

Ríos, R ;

Arce, VM ;

Devesa, J .

HORMONE RESEARCH, 2000, 53 (01) :40-45

← 1 2 3 →