Aspergillus oryzae palBory encodes a calpain-like protease:: Homology to Emericella nidulans PalB and conservation of functional regions

被引:12
作者
Futai, E
Sorimachi, H [1 ]
Jeong, S
Kitamoto, K
Ishiura, S
Suzuki, K
机构
[1] Univ Tokyo, Inst Mol & Cellular Biosci, Dept Mol Biol, Bunkyo Ku, Tokyo 1130032, Japan
[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan
[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Engn, Bunkyo Ku, Tokyo 1138657, Japan
[4] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan
关键词
Aspergillus oryzae; calpain; alkaline adaptation; PalB homologue;
D O I
10.1016/S1389-1723(99)80223-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have cloned and sequenced genomic DNA of Aspergillus oryzae palB(ory), orthologue of Emericella nidulans palB, which encodes a calpain-like protease modulating a signal transduction pathway during alkaline adaptation. The deduced amino acid sequence of PalB(ory) is 70.0% identical to PalB over its entire length. The regions with high similarity revealed possible domains important for their function. This is the first step towards understanding the alkaline adaptation mechanism of A. oryzae, which would be very useful in the fermentation industry.
引用
收藏
页码:438 / 440
页数:3
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