Purification and identification of adipogenic-differentiating peptides from egg white hydrolysate

被引：37

作者：

Jahandideh, Forough ^{[1
,2
]}

Liu, Ping ^{[1
]}

Wu, Jianping ^{[1
,2
]}

机构：

[1] Univ Alberta, Dept Agr Food & Nutr Sci, 4-10 Ag Ctr, Edmonton, AB T6G 2P5, Canada

[2] Univ Alberta, Cardiovasc Res Ctr, Edmonton, AB T6G 2P5, Canada

来源：

FOOD CHEMISTRY | 2018年 / 259卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Bioactive peptides; Egg white proteins; Adipogenic peptides; Liquid chromatography; RENIN-ANGIOTENSIN SYSTEM; HIGH-FAT DIET; ADIPOSE-TISSUE DYSFUNCTION; INSULIN-RESISTANCE; BIOACTIVE PEPTIDES; ADIPOCYTE DIFFERENTIATION; ALDOSTERONE SYSTEM; DIABETES-MELLITUS; TNF-ALPHA; PROTEIN;

D O I：

10.1016/j.foodchem.2018.03.099

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Egg proteins are a good source of bioactive peptides. Previous work from our research group has demonstrated the potential of egg white hydrolysate (EWH) for enhancing adipogenic differentiation and insulin signalling in 3T3-F442A pre-adipocytes. EWH was prepared by a combination of thermolysin and pepsin. Here, in this study, we aimed to identify the responsible peptide(s) in EWH. EWH was fractionated stepwise by ultrafiltration, C18 Sep-Pack cartridge, cation-exchange chromatography, and reverse-phase chromatography. The two most active fractions were analyzed by LC-MS/MS and 42 peptides were identified. Eleven peptides were synthesized and WEKAFKDED, QAMPFRVTEQE, ERYPIL, and VFKGL from ovalbumin were validated with peroxisome pro-liferator-associated receptor gamma stimulatory activity in adipocytes. For the first time, adipogenic differentiating peptides were characterized from egg white proteins. This data is pivotal for future structure-function studies of adipogenic peptides.

引用

页码：25 / 30

页数：6

共 37 条

[1] Oral administration of the milk casein-derived tripeptide Val-Pro-Pro attenuates high-fat diet-induced adipose tissue inflammation in mice [J].