Development of an aptamer-conjugated fluorescent nanoprobe for MMP2

被引：10

作者：

Han, Myoung-Eun ^{[1
,2
]}

Baek, Sungmin ^{[1
,2
]}

Kim, Hyun-Jung ^{[1
]}

Lee, Jung Hwan ^{[3
]}

Ryu, Sung-Ho ^{[3
]}

Oh, Sae-Ock ^{[1
,2
]}

机构：

[1] Pusan Natl Univ, Sch Med, Dept Anat, Yangsan 626870, South Korea

[2] Pusan Natl Univ, Med Res Ctr Ischem Tissue Regenerat, Yangsan 626870, South Korea

[3] Pohang Univ Sci & Technol, Med POSTECH Aptamer Initiat Program, Div Integrat Biosci & Biotechnol, Pohang 790784, South Korea

来源：

NANOSCALE RESEARCH LETTERS | 2014年 / 9卷

基金：

新加坡国家研究基金会;

关键词：

MMP2; Aptamer; Nanoprobe; Atherosclerosis; Cancer; MATRIX-METALLOPROTEINASE ACTIVITY; ATHEROSCLEROSIS; CANCER;

D O I：

10.1186/1556-276X-9-104

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Matrix metalloproteinase 2 (MMP2) plays critical roles in various diseases, such as atherosclerosis and cancer, and has been suggested to contribute to the instability of atherosclerotic plaque. To visualize MMP2 in pathologic tissues, we developed an aptamer targeting MMP2 protein by performing eight rounds of modified DNA systematic evolution of ligands by exponential enrichment (SELEX). The aptamer showed high affinity for MMP2 (K (d) = 5.59 nM), precipitated MMP2, and detected MMP2 protein in pathological tissues such as atherosclerotic plaque and gastric cancer tissues. Furthermore, a MMP2 aptamer-conjugated fluorescent nanoprobe successfully visualized atherosclerotic plaques in apolipoprotein E (ApoE) knockout mice. These results suggest that the devised MMP2 aptamer could be useful for the development of various diagnostic tools.

引用

页码：1 / 7

页数：7

共 18 条

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