QTL mapping of resistance to bacterial fruit blotch in Citrullus amarus

被引：17

作者：

Branham, Sandra E. ^{[1
]}

Levi, Amnon ^{[1
]}

Katawczik, Melanie L. ^{[1
]}

Wechter, W. Patrick ^{[1
]}

机构：

[1] ARS, US Vegetable Lab, USDA, 2700 Savannah Highway, Charleston, SC 29414 USA

来源：

THEORETICAL AND APPLIED GENETICS | 2019年 / 132卷 / 05期

基金：

美国农业部; 美国食品与农业研究所;

关键词：

AVENAE SUBSP CITRULLI; QUANTITATIVE TRAIT LOCI; NIVEUM RACE 2; DISEASE RESISTANCE; ACIDOVORAX-CITRULLI; GENETIC DIVERSITY; POWDERY MILDEW; WATERMELON; LANATUS; IDENTIFICATION;

D O I：

10.1007/s00122-019-03292-6

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Key messageSix QTLs were associated with affected leaf area in response to inoculation with Acidovorax citrulli in a recombinant inbred line population of Citrullus amarus.AbstractAcidovorax citrulli, the causal agent of bacterial fruit blotch (BFB) of cucurbits, has the potential to devastate production of watermelon and other cucurbits. Despite decades of research on host-plant resistance to A. citrulli, no germplasm has been found with immunity and only a few sources with various levels of BFB resistance have been identified, but the genetic basis of resistance in these watermelon sources are not known. Most sources of resistance are plant introductions of Citrullus amarus (citron melon), a closely related species that crosses readily with cultivated watermelon (Citrullus lanatus L.). In this study, we evaluated a recombinant inbred line population (N=200), derived from a cross between BFB-resistant (USVL246-FR2) and BFB-susceptible (USVL114) C. amarus lines, for foliar resistance to A. citrulli in three replicated greenhouse trials. We found the genetics of BFB resistance to be complicated by strong environmental influence, low heritability and significant genotype-by-environment interactions. QTL mapping of affected leaf area identified six QTL that each explained between 5 and 15% of the variation in BFB resistance in the population. This study represents the first identification of QTL associated with resistance to A. citrulli in any cucurbit.

引用

页码：1463 / 1471

页数：9

共 63 条

[11] Watermelon origin solved with molecular phylogenetics including Linnaean material: another example of museomics [J].

Chomicki, Guillaume ;

Renner, Susanne S. .

NEW PHYTOLOGIST, 2015, 205 (02) :526-532

[12]

Core Team R, 2018, R LANG ENV STAT COMP

[13]

CORRELL JC, 1994, PLANT DIS, V78, P653, DOI [10.1094/PD-78-0653, 10.1094/PD-78-0831C]

[14]

CRALL J M, 1969, Plant Disease Reporter, V53, P74

[15]

Demir G., 1996, Journal of Turkish Phytopathology, V25, P43

[16] Location of Acidovorax citrulli in Infested Watermelon Seeds Is Influenced by the Pathway of Bacterial Invasion [J].

Dutta, B. ;

Avci, U. ;

Hahn, M. G. ;

Walcott, R. R. .

PHYTOPATHOLOGY, 2012, 102 (05) :461-468

[17] A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species [J].

Elshire, Robert J. ;

Glaubitz, Jeffrey C. ;

Sun, Qi ;

Poland, Jesse A. ;

Kawamoto, Ken ;

Buckler, Edward S. ;

Mitchell, Sharon E. .

PLOS ONE, 2011, 6 (05)

[18]

Gilmore A.R., 2009, Asreml User Guide Release 3.0

[19] The epidemiology and management of seedborne bacterial diseases [J].

Gitaitis, Ronald ;

Walcott, Ronald .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 2007, 45 :371-397

[20] ERECTA, an LRR receptor-like kinase protein controlling development pleiotropically affects resistance to bacterial wilt [J].

Godiard, L ;

Sauviac, L ;

Torii, KU ;

Grenon, O ;

Mangin, B ;

Grimsley, NH ;

Marco, Y .

PLANT JOURNAL, 2003, 36 (03) :353-365

← 1 2 3 4 5 6 7 →