SC2: A novel process for manufacturing multipurpose high-density chemical microarrays

被引：47

作者：

Dikmans, Antonius ^{[1
]}

Beutling, Ulrike ^{[1
]}

Schmeisser, Edelgard ^{[1
]}

Thiele, Sabin ^{[1
]}

Frank, Ronald ^{[1
]}

机构：

[1] Helmholtz Ctr Infect Res, Dept Biol Chem, D-38124 Braunschweig, Germany

来源：

QSAR & COMBINATORIAL SCIENCE | 2006年 / 25卷 / 11期

关键词：

cellulose conjugates; chemical microarrays; diketopiperazine library; peptide library; small molecules;

D O I：

10.1002/qsar.200640130

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Various formats of miniaturized arrays of cellulose-bound synthetic probe molecules to suit a broad range of technical and assay configurations have been produced by a novel process which we call spotting compound-support conjugates, SC2. This process involves parallel chemical assembly of the probe molecules by SPOT-synthesis on a special cellulose membrane, post-synthesis separation of the tethered compound spots, solubilization of the cellulose matrix and spotting of the soluble cellulose-probe conjugates onto various types of planar surfaces. The performance of such arrays are demonstrated using a set of synthetic peptide analogues, assayed with a monoclonal antibody, as well as utilizing a small molecule library of diketopiperazines assayed with fluorescently labelled calmodulin.

引用

页码：1069 / 1080

页数：12

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