Molecular Identification of Gram-Negative Bacteria in Respiratory Samples of Cystic Fibrosis Patients from a Children Referral Hospital in Tehran

被引：3

作者：

Azimi, Leila ^{[1
]}

Ghanaiee, Roxana Mansour ^{[1
]}

Shirdust, Mehdi ^{[1
]}

Karimi, Ahmad Moein ^{[2
]}

Armin, Shahnaz ^{[1
]}

Tabatabaei, Sedigheh Rafiei ^{[1
]}

Shiva, Farideh ^{[1
]}

Fallah, Fatemeh ^{[1
]}

Rezaei, Mohammad ^{[3
]}

Fahimzad, Sayyed Alireza ^{[1
]}

Khanbabaei, Ghamartaj ^{[1
]}

Rahbar, Mohammad ^{[1
]}

机构：

[1] Shahid Beheshti Univ Med Sci, Pediat Infect Res Ctr, Res Inst Children Hlth, Tehran, Iran

[2] Islamic Azad Univ, Tehran Med Sci Branch, Tehran, Iran

[3] Alborz Univ Med Sci, Alborz, Iran

来源：

ARCHIVES OF PEDIATRIC INFECTIOUS DISEASES | 2019年 / 7卷 / 03期

关键词：

Pseudomonas; Cystic Fibrosis; Polymerase Chain Reaction; PSEUDOMONAS-AERUGINOSA; PATHOGENS; INFECTION;

D O I：

10.5812/pedinfect.64834

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

Background: Gram-negative bacteria are a major cause of pulmonary infection in patients with cystic fibrosis (CF). Objectives: The study aimed to conduct the molecular identification of Gram-negative bacterial flora causing pulmonary infection in children with CF. Methods: In this study, sputum samples were taken from 64 CF children undergoing treatment as outpatients or inpatients at a referral children's hospital in Tehran. The PCR technique was used to detect the presence of Gram-negative bacteria, namely Pseudomonas spp., Acinetobacter baumannii, Stenotrophomonas maltophilia, and Burkholderia cepacia. Results: All samples were positive for 16srRNA. Pseudomonas spp. and A. baumannii were detected in 47% and 14% of the studied samples, respectively. Co-colonization by Pseudomonas spp. and A. baumannii was observed in three (5%) samples. Conclusions: According to this survey, Pseudomonas spp. were the most prevalent Gram-negative bacteria isolated from CF patients with pulmonary infection by molecular assays.

引用

页数：5

共 27 条

[1]

Anbazhagan D, 2011, BRAZ J MICROBIOL, V42, P448, DOI 10.1590/S1517-83822011000200006

[2] Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis from Burkholderia cepacia genomovars I, III, and IV by PCR [J].