共 23 条
A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus
被引:21
作者:
Li, Ruichao
[1
,2
]
Chiou, Jiachi
[1
,2
]
Chan, Edward Wai-Chi
[1
,2
]
Chen, Sheng
[1
,2
]
机构:
[1] Hong Kong PolyU Shen Zhen Res Inst, Food Safety & Technol Res Ctr, Shenzhen Key Lab Food Biol Safety Control, Shenzhen, Peoples R China
[2] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, State Key Lab Chirosci, Hong Kong, Hong Kong, Peoples R China
来源:
FRONTIERS IN MICROBIOLOGY
|
2016年
/
7卷
关键词:
Vibrio parahaemolyticus;
bla(CARB-17);
molecular detection;
PCR;
MULTIPLEX PCR;
CHOLERAE;
STRAINS;
GENE;
VULNIFICUS;
ASSAY;
AMPLIFICATION;
SHELLFISH;
TDH;
D O I:
10.3389/fmicb.2016.00044
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the bla(CARB-17) like element, an intrinsic beta-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that bla(CARB-17) like genes were more conservative than the tlh. toxR and atpA genes, the genetic markers commonly used as detection targets in identification of V parahaemolyticus. Our data showed that this bla(CARB-17)-specificPCR-based detection approach consistently achieved 100% specificity, whereas PCR targeting the tlh and atpA genes occasionally produced false positive results. Furthermore, a positive result of this test is consistently associated with an intrinsic ampicillin resistance phenotype of the test organism, presumably conferred by the products of bla(CARB-17) like genes. We envision that combined analysis of the unique genetic and phenotypic characteristics conferred by bla(CARB-17) shall further enhance the detection specificity of this novel yet easy-to-use detection approach to a level superior to the conventional methods used in V parahaemolyticus detection and identification.
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