A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus

被引:21
作者
Li, Ruichao [1 ,2 ]
Chiou, Jiachi [1 ,2 ]
Chan, Edward Wai-Chi [1 ,2 ]
Chen, Sheng [1 ,2 ]
机构
[1] Hong Kong PolyU Shen Zhen Res Inst, Food Safety & Technol Res Ctr, Shenzhen Key Lab Food Biol Safety Control, Shenzhen, Peoples R China
[2] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, State Key Lab Chirosci, Hong Kong, Hong Kong, Peoples R China
来源
FRONTIERS IN MICROBIOLOGY | 2016年 / 7卷
关键词
Vibrio parahaemolyticus; bla(CARB-17); molecular detection; PCR; MULTIPLEX PCR; CHOLERAE; STRAINS; GENE; VULNIFICUS; ASSAY; AMPLIFICATION; SHELLFISH; TDH;
D O I
10.3389/fmicb.2016.00044
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the bla(CARB-17) like element, an intrinsic beta-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that bla(CARB-17) like genes were more conservative than the tlh. toxR and atpA genes, the genetic markers commonly used as detection targets in identification of V parahaemolyticus. Our data showed that this bla(CARB-17)-specificPCR-based detection approach consistently achieved 100% specificity, whereas PCR targeting the tlh and atpA genes occasionally produced false positive results. Furthermore, a positive result of this test is consistently associated with an intrinsic ampicillin resistance phenotype of the test organism, presumably conferred by the products of bla(CARB-17) like genes. We envision that combined analysis of the unique genetic and phenotypic characteristics conferred by bla(CARB-17) shall further enhance the detection specificity of this novel yet easy-to-use detection approach to a level superior to the conventional methods used in V parahaemolyticus detection and identification.
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页数:5
相关论文
共 23 条
[1]   Vibrios as causal agents of zoonoses [J].
Austin, B. .
VETERINARY MICROBIOLOGY, 2010, 140 (3-4) :310-317
[2]   A novel multiplex PCR for the identification of Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus [J].
Bauer, A. ;
Rorvik, L. M. .
LETTERS IN APPLIED MICROBIOLOGY, 2007, 45 (04) :371-375
[3]   Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh [J].
Bej, AK ;
Patterson, DP ;
Brasher, CW ;
Vickery, MCL ;
Jones, DD ;
Kaysner, CA .
JOURNAL OF MICROBIOLOGICAL METHODS, 1999, 36 (03) :215-225
[4]   CARB-17 Family of β-Lactamases Mediates Intrinsic Resistance to Penicillins in Vibrio parahaemolyticus [J].
Chiou, Jiachi ;
Li, Ruichao ;
Chen, Sheng .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2015, 59 (06) :3593-3595
[5]   Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus [J].
Di Pinto, Angela ;
Terio, Valentina ;
Novello, Lucia ;
Tantillo, Giuseppina .
FOOD CONTROL, 2011, 22 (01) :124-127
[6]   Quantitative Microbial Risk Assessment of Pathogenic Vibrios in Marine Recreational Waters of Southern California [J].
Dickinson, Gregory ;
Lim, Keah-Ying ;
Jiang, Sunny C. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (01) :294-302
[7]   Genome Sequence of the Clinical O4:K12 Serotype Vibrio parahaemolyticus Strain 10329 [J].
Gonzalez-Escalona, N. ;
Strain, E. A. ;
De Jesus, A. J. ;
Jones, J. L. ;
DePaola, A. .
JOURNAL OF BACTERIOLOGY, 2011, 193 (13) :3405-3406
[8]   Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus [J].
Izumiya, Hidemasa ;
Matsumoto, Kazutoshi ;
Yahiro, Shunsuke ;
Lee, Jiyoung ;
Morita, Masatomo ;
Yamamoto, Shouji ;
Arakawa, Eiji ;
Ohnishi, Makoto .
MOLECULAR AND CELLULAR PROBES, 2011, 25 (04) :174-176
[9]   Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene [J].
Kim, YB ;
Okuda, J ;
Matsumoto, C ;
Takahashi, N ;
Hashimoto, S ;
Nishibuchi, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (04) :1173-1177
[10]   Genes Similar to the Vibrio parahaemolyticus Virulence-Related Genes tdh, tlh, and vscC2 Occur in Other Vibrionaceae Species Isolated from a Pristine Estuary [J].
Klein, Savannah L. ;
West, Casandra K. Gutierrez ;
Mejia, Diana M. ;
Lovell, Charles R. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014, 80 (02) :595-602