Intertribal somatic hybrids between Brassica napus and Camelina sativa with high linolenic acid content

被引：16
|
作者：
Jiang, J. J. [1 ]
Zhao, X. X. [2 ]
Tian, W. [1 ]
Li, T. B. [1 ]
Wang, Y. P. [1 ]
机构：
[1] Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China
[2] Huaiyin Normal Coll, Biotechnol Key Lab Ecoagr Hongze Lake Reg, Huaian 223001, Peoples R China
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2009年 / 99卷 / 01期
关键词：
Brassica napus; Camelina sativa; Protoplast fusion; Fatty acid alternation; SEQUENCE REPEATS SSRS; FATTY-ACIDS; RAPESEED; BIOSYNTHESIS;
D O I：
10.1007/s11240-009-9579-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Intertribal somatic hybrids of Brassica napus and Camelina sativa were developed by protoplast electrofusion. Hybrid identity of the regenerants was determined using flow cytometric analysis of nuclear DNA content and simple sequence repeat (SSR) marker analysis. Three hybrids exhibited specific bands for B. napus and C. sativa. These hybrids showed intermediate leaf, flower and seed morphology compared with the two parental species. The seeds of these three hybrids had a modified fatty acid profile, indicating higher level of linolenic and eicosanoic acids than those of B. napus. Our results suggest that somatic hybridization offers opportunities for transferring entire genomes between B. napus and C. sativa in improving rapeseed breeding.
引用
收藏
页码：91 / 95
页数：5
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