Chondrogenic properties of collagen type XI, a component of cartilage extracellular matrix

被引：50
作者：
Li, Ang [1 ]
Wei, Yiyong [1 ]
Hung, Clark [1 ]
Vunjak-Novakovic, Gordana [1 ,2 ]
机构：
[1] Columbia Univ, Dept Biomed Engn, New York, NY 10027 USA
[2] Columbia Univ, Dept Med, New York, NY USA
来源：
BIOMATERIALS | 2018年 / 173卷
关键词：
Cartilage extracellular matrix; Human mesenchymal stem cells; Collagen type XI; Chondrogenesis; Cartilage matrix degradation; MESENCHYMAL STEM-CELLS; DOMAIN RECEPTOR 2; ARTICULAR-CARTILAGE; IN-VITRO; GROWTH; OSTEOARTHRITIS; CHONDROCYTES; DIFFERENTIATION; DEGRADATION; HYDROGELS;
D O I：
10.1016/j.biomaterials.2018.05.004
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Cartilage extracellular matrix (ECM) has been used for promoting tissue engineering. However, the exact effects of ECM on chondrogenesis and the acting mechanisms are not well understood. In this study, we investigated the chondrogenic effects of cartilage ECM on human mesenchymal stem cells (MSCs) and identified the contributing molecular components. To this end, a preparation of articular cartilage ECM was supplemented to pellets of chondrogenically differentiating MSCs, pellets of human chondrocytes, and bovine articular cartilage explants to evaluate the effects on cell proliferation and the production of cartilaginous matrix. Selective enzymatic digestion and screening of ECM components were conducted to identify matrix molecules with chondrogenic properties. Cartilage ECM promoted MSC proliferation, production of cartilaginous matrix, and maturity of chondrogenic differentiation, and inhibited the hypertrophic differentiation of MSC-derived chondrocytes. Selective digestion of ECM components revealed a contributory role of collagens in promoting chondrogenesis. The screening of various collagen subtypes revealed strong chondrogenic effect of collagen type XI. Finally, collagen XI was found to promote production and inhibit degradation of cartilage matrix in human articular chondrocyte pellets and bovine articular cartilage explants. Our results indicate that cartilage ECM promotes chondrogenesis and inhibits hypertrophic differentiation in MSCs. Collagen type Xl is the ECM component that has the strongest effects on enhancing the production and inhibiting the degradation of cartilage matrix. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码：47 / 57
页数：11
相关论文
共 45 条
[1]   Novel pathogenic COL11A1/COL11A2 variants in Stickler syndrome detected by targeted NGS and exome sequencing [J].
Acke, Frederic R. ;
Malfait, Fransiska ;
Vanakker, Olivier M. ;
Steyaert, Wouter ;
De Leeneer, Kim ;
Mortier, Geert ;
Dhooge, Ingeborg ;
De Paepe, Anne ;
De Leenheer, Els M. R. ;
Coucke, Paul J. .
MOLECULAR GENETICS AND METABOLISM, 2014, 113 (03) :230-235
[2]   Hyaluronic acid facilitates chondrogenesis and matrix deposition of human adipose derived mesenchymal stem cells and human chondrocytes co-cultures [J].
Amann, Elisabeth ;
Wolff, Paul ;
Breel, Ernst ;
van Griensven, Martijn ;
Balmayor, Elizabeth R. .
ACTA BIOMATERIALIA, 2017, 52 :130-144
[3]   Effects of Chondroitinase ABC-Mediated Proteoglycan Digestion on Decellularization and Recellularization of Articular Cartilage [J].
Bautista, Catherine A. ;
Park, Hee Jun ;
Mazur, Courtney M. ;
Aaron, Roy K. ;
Bilgen, Bahar .
PLOS ONE, 2016, 11 (07)
[4]  
Bhumiratana S., P NATL ACAD SCI US, V111
[5]   Collagen XI nucleates self-assembly and limits lateral growth of cartilage fibrils [J].
Blaschke, UK ;
Eikenberry, EF ;
Hulmes, DJS ;
Galla, HJ ;
Bruckner, P .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (14) :10370-10378
[6]   Hydrogels derived from cartilage matrices promote induction of human mesenchymal stem cell chondrogenic differentiation [J].
Burnsed, Olivia A. ;
Schwartz, Zvi ;
Marchand, Katherine O. ;
Hyzy, Sharon L. ;
Olivares-Navarrete, Rene ;
Boyan, Barbara D. .
ACTA BIOMATERIALIA, 2016, 43 :139-149
[7]   Crystallographic Insight into Collagen Recognition by Discoidin Domain Receptor 2 [J].
Carafoli, Federico ;
Bihan, Dominique ;
Stathopoulos, Stavros ;
Konitsiotis, Antonios D. ;
Kvansakul, Marc ;
Farndale, Richard W. ;
Leitinger, Birgit ;
Hohenester, Erhard .
STRUCTURE, 2009, 17 (12) :1573-1581
[8]   The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes [J].
Choi, Kyoung-Hwan ;
Choi, Byung Hyune ;
Park, So Ra ;
Kim, Byoung Ju ;
Min, Byoung-Hyun .
BIOMATERIALS, 2010, 31 (20) :5355-5365
[9]   An overview of tissue and whole organ decellularization processes [J].
Crapo, Peter M. ;
Gilbert, Thomas W. ;
Badylak, Stephen F. .
BIOMATERIALS, 2011, 32 (12) :3233-3243
[10]   A small-molecule inhibitor of the Wnt pathway (SM04690) as a potential disease modifying agent for the treatment of osteoarthritis of the knee [J].
Deshmukh, V. ;
Hu, H. ;
Barroga, C. ;
Bossard, C. ;
Kc, S. ;
Dellamary, L. ;
Stewart, J. ;
Chiu, K. ;
Ibanez, M. ;
Pedraza, M. ;
Seo, T. ;
Do, L. ;
Cho, S. ;
Cahiwat, J. ;
Tam, B. ;
Tambiah, J. R. S. ;
Hood, J. ;
Lane, N. E. ;
Yazici, Y. .
OSTEOARTHRITIS AND CARTILAGE, 2018, 26 (01) :18-27
12345