Homocysteine thiolactone is a positive effector of σS levels in Escherichia coli

被引:14
作者
Goodrich-Blair, H [1 ]
Kolter, R
机构
[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
[2] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
关键词
stationary phase; folate stress; metabolic signal; sigma factor;
D O I
10.1016/S0378-1097(00)00080-X
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
sigma(S) is a regulator of the stationary phase response in Escherichia coli. Multi-copy suppressors were sought in a strain with decreased levels of sigma(S) and one such suppressor was found to encode HsrA, a putative efflux pump. Multi-copy expression of hsrA was shown to lead to accumulation of homocysteine, which is predicted to cause an increase in homocysteine thiolactone (HCTL) levels. A direct correlation between HCTL levels and sigma(S) accumulation was observed both in mutants and during normal cell growth, leading to the hypothesis that HCTL is a physiologically relevant positive effector of sigma(S) levels in vivo. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:117 / 121
页数:5
相关论文
共 18 条
[1]   THE DPS PROMOTER IS ACTIVATED BY OXYR DURING GROWTH AND BY IHF AND A SIGMA(S) IN STATIONARY-PHASE [J].
ALTUVIA, S ;
ALMIRON, M ;
HUISMAN, G ;
KOLTER, R ;
STORZ, G .
MOLECULAR MICROBIOLOGY, 1994, 13 (02) :265-272
[2]   FUNCTIONALLY DIVERSE ENZYME SUPERFAMILY THAT ABSTRACTS THE ALPHA-PROTONS OF CARBOXYLIC-ACIDS [J].
BABBITT, PC ;
MRACHKO, GT ;
HASSON, MS ;
HUISMAN, GW ;
KOLTER, R ;
RINGE, D ;
PETSKO, GA ;
KENYON, GL ;
GERLT, JA .
SCIENCE, 1995, 267 (5201) :1159-1161
[3]   THE M-GAMMA-DELTA-1 ELEMENT, A SMALL GAMMA-DELTA (TN1000) DERIVATIVE USEFUL FOR PLASMID MUTAGENESIS, ALLELE REPLACEMENT AND DNA SEQUENCING [J].
BERG, CM ;
VARTAK, NB ;
WANG, G ;
XU, XX ;
LIU, L ;
MACNEIL, DJ ;
GEWAIN, KM ;
WIATER, LA ;
BERG, DE .
GENE, 1992, 113 (01) :9-16
[4]  
BOGNAR AL, 1985, J BIOL CHEM, V260, P5625
[5]   STATIONARY-PHASE-INDUCIBLE GEARBOX PROMOTERS - DIFFERENTIAL-EFFECTS OF KATF MUTATIONS AND ROLE OF SIGMA-70 [J].
BOHANNON, DE ;
CONNELL, N ;
KEENER, J ;
TORMO, A ;
ESPINOSAURGEL, M ;
ZAMBRANO, MM ;
KOLTER, R .
JOURNAL OF BACTERIOLOGY, 1991, 173 (14) :4482-4492
[6]   DNA-SEQUENCE AND ANALYSIS OF 136 KILOBASES OF THE ESCHERICHIA-COLI GENOME - ORGANIZATIONAL SYMMETRY AROUND THE ORIGIN OF REPLICATION [J].
BURLAND, V ;
PLUNKETT, G ;
DANIELS, DL ;
BLATTNER, FR .
GENOMICS, 1993, 16 (03) :551-561
[7]   MUTANTS OF ESCHERICHIA-COLI REQUIRING METHIONINE OR VITAMIN-B12 [J].
DAVIS, BD ;
MINGIOLI, ES .
JOURNAL OF BACTERIOLOGY, 1950, 60 (01) :17-28
[8]  
Goodrich-Blair Heidi, 1996, P571
[9]  
Greene RC., 1996, Escherichia coli and Salmonella, P542
[10]   SENSING STARVATION - A HOMOSERINE LACTONE-DEPENDENT SIGNALING PATHWAY IN ESCHERICHIA-COLI [J].
HUISMAN, GW ;
KOLTER, R .
SCIENCE, 1994, 265 (5171) :537-539