Optimisation of combi-lipases from Aspergillus niger for the synergistic and efficient hydrolysis of soybean oil

被引：34

作者：

Qiao, Hanzhen ^{[1
,2
]}

Zhang, Fei ^{[1
,2
]}

Guan, Wutai ^{[1
,2
]}

Zuo, Jianjun ^{[1
]}

Feng, Dingyuan ^{[1
]}

机构：

[1] South China Agr Univ, SCAU UniOil Feeding Oil & Fat Res Ctr, Guangzhou, Guangdong, Peoples R China

[2] South China Agr Univ, Coll Anim Sci, Wushan Ave, Guangzhou, Guangdong, Peoples R China

来源：

ANIMAL SCIENCE JOURNAL | 2017年 / 88卷 / 05期

关键词：

animal feed; combi-lipase; fatty acid; hydrolysis; soybean oil; BIODIESEL PRODUCTION; PICHIA-PASTORIS; FUNGAL LIPASE; PURIFICATION; BIOCATALYSTS; DIGESTION; MIXTURES; ENZYMES; SYSTEM;

D O I：

10.1111/asj.12718

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The enzymatic properties of four lipases (A, B, C and D) from different strains of Aspergillus niger, were investigated, and a 3-factor mixture design and triangular surface analysis were performed to screen the optimal combi-lipase by observing synergistic effects. Lipases B and D differed in optimal pH, temperature and substrate specificity. A combi-lipase with 31.2% lipase B and 68.8% lipase D (w/w, equal to units of 30.36% and 69.64%) exhibited optimal hydrolytic activity on soybean oil, which exceeded the sum of the combined activities of individual lipases (P<0.05). Free fatty acid from the hydrolyzed soybean oil indicated that the synergistic effect of the combi-lipase resulted from the different fatty acid specificities of the two lipases. Overall, combi-lipase afforded an effective route for the application of lipase enzymes to animal feeds.

引用

页码：772 / 780

页数：9

共 40 条

[1] Combi-lipase for heterogeneous substrates: a new approach for hydrolysis of soybean oil using mixtures of biocatalysts [J].