Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modeling of its fermentation in shake flasks

被引：17

作者：

Munawar, Khalil Munawar Makhdum ^{[1
]}

Simarani, Khanom ^{[1
]}

Annuar, Mohamad Suffian Mohamad ^{[1
]}

机构：

[1] Univ Malaya, Inst Biol Sci, Fac Sci, Kuala Lumpur, Malaysia

来源：

ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2016年 / 19卷

关键词：

Biopolymer; Fermentation; Medium-chain-length PHA; Palm kernel oil; Pseudomonas putida; EXTRACELLULAR LIPASE; PURIFICATION;

D O I：

10.1016/j.ejbt.2015.07.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: The paper reports on the utilization of palm kernel oil (PKO) as a low cost renewable substrate for medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) production by Pseudomonas putida BET001. Investigation on the effects of selected key variables on growth, mixed free fatty acids consumption and mcl-PHA production by the bacterial culture in the shaken flask system were carried out along with its kinetic modeling. Results: The biomass production, fatty acids consumption and mcl-PHA production were found favorable when the strain was cultured in mineral medium at pH 6-7, 28 degrees C, aeration surface-to-volume ratio of 0.4 x 10(6)m(-1), 250 rpm agitation rate for 48 h. Mcl-PHA production by this strain showed mixed growth and non-growth associated components as described by Luedeking-Piret kinetic model. Conclusion: The findings of this study provided add to the literature on key variables in for achieving good microbial growth andmcl-PHA production in shake flasks culture. In addition, suitable kinetic model to describe cultivation in this system was also presented. (C) 2015 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.

引用

页码：50 / 55

页数：6

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