Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

被引：75

作者：

Ueda, Jun ^{[1
,2
]}

Harada, Akihito ^{[3
]}

Urahama, Takashi ^{[4
]}

Machida, Shinichi ^{[4
]}

Maehara, Kazumitsu ^{[3
]}

Hada, Masashi ^{[5
]}

Makino, Yoshinori ^{[5
]}

Nogami, Jumpei ^{[3
]}

Horikoshi, Naoki ^{[4
]}

Osakabe, Akihisa ^{[4
]}

Taguchi, Hiroyuki ^{[4
]}

Tanaka, Hiroki ^{[4
]}

Tachiwana, Hiroaki ^{[4
]}

Yao, Tatsuma ^{[6
,9
]}

Yamada, Minami ^{[1
]}

Iwamoto, Takashi ^{[1
]}

Isotani, Ayako ^{[2
]}

Ikawa, Masahito ^{[2
]}

Tachibana, Taro ^{[7
]}

Okada, Yuki ^{[5
]}

Kimura, Hiroshi ^{[8
]}

Ohkawa, Yasuyuki ^{[3
]}

Kurumizaka, Hitoshi ^{[4
]}

Yamagata, Kazuo ^{[2
,9
]}

机构：

[1] Chubu Univ, Ctr Educ Lab Anim Res, Kasugai, Aichi 4878501, Japan

[2] Osaka Univ, Microbial Dis Res Inst, Ctr Genet Anal Biol Responses, 3-1 Yamadaoka, Suita, Osaka 5650871, Japan

[3] Kyushu Univ, Med Inst Bioregulat, Div Transcript, Fukuoka 8128582, Japan

[4] Waseda Univ, Grad Sch Adv Sci & Engn, Struct Biol Lab, Tokyo 1628480, Japan

[5] Univ Tokyo, Inst Mol & Cellular Biosci, Lab Pathol & Dev, Tokyo 1130032, Japan

[6] Fuso Pharmaceut Ind Ltd, Ctr Res & Dev, Osaka 5368523, Japan

[7] Osaka City Univ, Grad Sch Engn, Dept Bioengn, Osaka 5588585, Japan

[8] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Midori Ku, 4259 Nagatsuta Cho, Yokohama, Kanagawa 2268501, Japan

[9] Kindai Univ, Fac Biol Oriented Sci & Technol, Wakayama 6496493, Japan

来源：

CELL REPORTS | 2017年 / 18卷 / 03期

基金：

日本学术振兴会;

关键词：

RETINOIC ACID; MOUSE TESTIS; STEM-CELLS; NUCLEOSOME; CHROMATIN; H3.3; EXPRESSION; GENERATION; TH2B;

D O I：

10.1016/j.celrep.2016.12.065

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-typespecific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

引用

页码：593 / 600

页数：8

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