Tol2 transposon-mediated transgenesis in Xenopus tropicalis

被引:79
作者
Hamlet, Michelle R. Johnson
Yergeau, Donald A.
Kuliyev, Emin
Takeda, Masatoshi
Taira, Masanori
Kawakami, Koichi
Mead, Paul E.
机构
[1] St Jude Childrens Hosp, Dept Pathol, Memphis, TN 38105 USA
[2] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo, Japan
[3] Natl Inst Genet, Div Mol & Dev Biol, Shizuoka, Japan
关键词
transposon; Xenopus tropicalis; Tol2; transgenics;
D O I
10.1002/dvg.20234
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The diploid frog Xenopus tropicalis is becoming a powerful developmental genetic model system. Sequencing of the X tropicalis genome is nearing completion and several labs are embarking on mutagenesis screens. We are interested in developing insertional mutagenesis strategies in X tropicalis. Transposon-mediated insertional mutagenesis, once used exclusively in plants and invertebrate systems, is now more widely applicable to vertebrates. The first step in developing transposons as tools for mutagenesis is to demonstrate that these mobile elements function efficiently in the target organism. Here, we show that the Medaka fish transposon, Tol2, is able to stably integrate into the X. tropicalis genome and will serve as a powerful tool for insertional mutagenesis strategies in the frog.
引用
收藏
页码:438 / 445
页数:8
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