High performance characterization of triacylglycerols in milk and milk-related samples by liquid chromatography and mass spectrometry

被引：54

作者：

Beccaria, Marco ^{[1
]}

Sullini, Giuseppe ^{[1
]}

Cacciola, Francesco ^{[2
,3
]}

Donato, Paola ^{[1
,3
,4
]}

Dugo, Paola ^{[1
,3
,4
]}

Mondello, Luigi ^{[1
,3
,4
]}

机构：

[1] Univ Messina, Dipartimento Sci Farmaco & Prodotti Salute SCIFAR, I-98168 Messina, Italy

[2] Univ Messina, Dipartimento Sci Ambiente Sicurezza Terr Alimenti, I-98166 Messina, Italy

[3] Univ Messina, Dipartimento Sci Farmaco & Prodotti Salute SCIFAR, Chromaleont Srl, I-98168 Messina, Italy

[4] Univ Campus Biomed Rome, Ctr Integrato Ric, I-00128 Rome, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2014年 / 1360卷

关键词：

Milk; Triacylglycerols; Mass spectrometry; Serially-coupled columns; Fused-core; EVAPORATIVE LIGHT-SCATTERING; NONAQUEOUS REVERSED-PHASE; SILVER-ION; FATTY-ACID; PEAK-CAPACITY; DONKEY MILK; SEPARATION; ELUCIDATION; PROFILES; LIPIDS;

D O I：

10.1016/j.chroma.2014.07.073

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, ultra high performance liquid chromatography was used for the characterization of non polar lipids (triacylglycerols) in milk samples of different origin, as well as milk-derivatives. For tackling such a task, three core-shell type octadecylsilica columns were serially coupled, reaching a total stationary Phase length of 45 cm, using acetonitrile-isopropanol gradient elution allowing triacylglicerol separation according to increasing partition number. The employment of an ion-trap-time-of-flight detection in conjunction with atmospheric-pressure chemical ionization mass spectrometry was carried out to positively identify a number of 243 different triacylglycerols containing up to 22 fatty acids, with 2-22 carbon atom alkyl chain length, and 0-3 double bonds. This work reports an extensive characterization of the triacylglycerol fraction in milk and milk-related samples of different sources. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：172 / 187

页数：16

共 44 条

[1] Simple complementary liquid chromatography and mass spectrometry approaches for the characterization of triacylglycerols in Pinus koraiensis seed oil [J].