Spore Display Using Bacillus thuringiensis Exosporium Protein InhA

被引：12

作者：

Park, Tae Jung ^{[2
,3
]}

Choi, Soo-Keun ^{[1
]}

Jung, Heung-Chae ^{[1
]}

Lee, Sang Yup ^{[2
,3
,4
,5
]}

Pan, Jae-Gu ^{[1
,6
]}

机构：

[1] GenoFocus, Natl Lab Microbial Display, Taejon 305811, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Program BK21, BioProc Engn Res Ctr,Ctr Syst & Synthet Biotechno, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Inst BioCentury, Taejon 305701, South Korea

[4] Korea Adv Inst Sci & Technol, Dept Bio & Brain Engn, Taejon 305701, South Korea

[5] Korea Adv Inst Sci & Technol, Bioinformat Res Ctr, Taejon 305701, South Korea

[6] Korea Res Inst Biosci & Biotechnol, Syst Microbiol Res Ctr, Taejon 305806, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2009年 / 19卷 / 05期

关键词：

Bacillus thuringiensis; spore display; exosporium; InhA; BACTERIAL SURFACE DISPLAY; SUBTILIS SPORES; CELL-SURFACE; HETEROLOGOUS PROTEINS; ANTHRACIS EXOSPORIUM; METALLOPROTEASE; IDENTIFICATION; LIBRARIES; ANTIGENS; CEREUS;

D O I：

10.4014/jmb.0802.163

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and P-galactosidase as model proteins were fused to the C-terminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display.

引用

页码：495 / 501

页数：7

共 50 条

[11] The ExsA protein of Bacillus cereus is required for assembly of coat and exosporium onto the spore surface
Bailey-Smith, K
Todd, SJ
Southworth, TW
Proctor, J
Moir, A
JOURNAL OF BACTERIOLOGY, 2005, 187 (11) : 3800 - 3806
[12] Role of novel polysaccharide layers in assembly of the exosporium, the outermost protein layer of the Bacillus anthracis spore
Lehmann, Dorte
Sladek, Margaret
Khemmani, Mark
Boone, Tyler J.
Rees, Eric
Driks, Adam
MOLECULAR MICROBIOLOGY, 2022, 118 (03) : 258 - 277
[13] EXOSPORIUM AND SPORE COAT FORMATION IN BACILLUS-CEREUS-T
OHYE, DF
MURRELL, WG
JOURNAL OF BACTERIOLOGY, 1973, 115 (03) : 1179 - 1190
[14] Expression and characterization of inhA gene from Bacillus thuringiensis 8010
Xiaomin Yu
Tianpei Huang
Zhipeng Huang
Charles A. Powell
Xiong Guan
World Journal of Microbiology and Biotechnology, 2007, 23 : 1621 - 1625
[15] RELATIONSHIP OF THE SYNTHESES OF SPORE COAT PROTEIN AND PARASPORAL CRYSTAL PROTEIN IN BACILLUS-THURINGIENSIS
ARONSON, AI
TYRELL, DJ
FITZJAMES, PC
BULLA, LA
JOURNAL OF BACTERIOLOGY, 1982, 151 (01) : 399 - 410
[16] ANALYSIS OF A PROTEIN FRACTION IN SPORE COATS OF BACILLUS-THURINGIENSIS - COMPARISON WITH CRYSTAL PROTEIN
LECADET, MM
CHEVRIER, G
DEDONDER, R
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1972, 25 (02): : 349 - &
[17] Expression and characterization of inhA gene from Bacillus thuringiensis 8010
Yu, Xiaomin
Huang, Tianpei
Huang, Zhipeng
Powell, Charles A.
Guan, Xiong
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2007, 23 (11): : 1621 - 1625
[18] Display of Escherichia coli Phytase on the Surface of Bacillus subtilis Spore Using CotG as an Anchor Protein
Sirima Mingmongkolchai
Watanalai Panbangred
Applied Biochemistry and Biotechnology, 2019, 187 : 838 - 855
[19] Display of Escherichia coli Phytase on the Surface of Bacillus subtilis Spore Using CotG as an Anchor Protein
Mingmongkolchai, Sirima
Panbangred, Watanalai
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2019, 187 (03) : 838 - 855
[20] Bacillus subtilis spore display system
Kim, Junehyung
Kim, Byung-Gee
JOURNAL OF BIOTECHNOLOGY, 2008, 136 : S331 - S331

← 1 2 3 4 5 →