Hemocyanin-derived phenoloxidase reaction products display anti-infective properties

被引：33
作者：
Coates, Christopher J. [1 ]
Talbot, James [1 ]
机构：
[1] Swansea Univ, Coll Sci, Dept Biosci, Swansea SA2 8PP, W Glam, Wales
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2018年 / 86卷
关键词：
Horseshoe crabs; Innate immunity; Melanogenesis; Enzyme-substrate complexes; Multifunctional protein; GENERATED IN-VITRO; ANTIMICROBIAL ACTIVITY; TARANTULA HEMOCYANIN; LIMULUS-POLYPHEMUS; OXIDASE ACTIVATION; IMMUNE-RESPONSE; PROTEINS; CRAYFISH; MELANIN; LINK;
D O I：
10.1016/j.dci.2018.04.017
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Hemocyanin is a multi-functional protein located in the hemolymph (blood) of certain arthropods and molluscs. In addition to its well-defined role in oxygen transport, hemocyanin can be converted into a phenoloxidase-like enzyme. Herein, we tested the antimicrobial properties of horseshoe crab (Limulus polyphemus) hemocyanin-derived phenoloxidase reaction products using broad ranges of phenolic substrates (e.g. (L)-DOPA) and microbial targets (Gram-positive/negative bacteria, yeast). The enzyme catalysed turnover of several substrates generated (by)products that reduced significantly the number of colony forming units. Microbicidal effects of hemocyanin-derived phenoloxidase were thwarted by the inhibitor phenylthiourea. Data presented here further support a role for hemocyanin in invertebrate innate immunity. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码：47 / 51
页数：5
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