Visual Detection of DNA on Paper Chips

被引：42

作者：

Song, Yajing ^{[1
]}

Gyarmati, Peter ^{[1
]}

Araujo, Ana Catarina ^{[2
]}

Lundeberg, Joakim ^{[1
]}

Brumer, Harry, III ^{[2
,3
,4
]}

Stahl, Patrik L. ^{[5
]}

机构：

[1] Royal Inst Technol KTH, Sch Biotechnol, Sci Life Lab, Div Gene Technol, SE-17165 Solna, Sweden

[2] AlbaNova Univ Ctr, Royal Inst Technol KTH, Div Glycosci, Sch Biotechnol, SE-10691 Stockholm, Sweden

[3] Univ British Columbia, Michael Smith Labs, Vancouver, BC V167T 1Z4, Canada

[4] Univ British Columbia, Dept Chem, Vancouver, BC V167T 1Z4, Canada

[5] Karolinska Inst, Dept Cell & Mol Biol, SE-17177 Solna, Sweden

来源：

ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 03期

基金：

瑞典研究理事会; 加拿大自然科学与工程研究理事会;

关键词：

NUCLEIC-ACID AMPLIFICATION; IMMOBILIZED QUANTUM DOTS; HYBRIDIZATION ASSAY; REAL-TIME; MICROARRAYS; DEVICES; DIAGNOSTICS; DONORS;

D O I：

10.1021/ac403196b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

On-site DNA analysis for diagnostic or forensic purposes is much anticipated in the future of molecular testing. Yet the challenges to achieve this goal remain large with rapid and inexpensive detection and visualization being key factors for any portable analysis system. We have developed a filter paper-based nucleic acid assay, which is able to identify and distinguish dog and human genomic and mitochondrial samples in a forensic setting. The filter paper material allows for transport by capillary force of the sample DNA through the detection surface, allowing the targets to hybridize specifically to their complementary capture sequences. Coupling micrometer-sized beads to DNA allows the results to be visualized by the naked eye, enabling instant, cost-efficient, and on-site detection, while eliminating the need for advanced expensive instrumentation.

引用

页码：1575 / 1582

页数：8

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