Enhanced gellan gum production by hydrogen peroxide (H2O2) induced oxidative stresses in Sphingomonas paucimobilis

被引:8
|
作者
Zhu, Guilan [1 ,2 ]
Sheng, Long [2 ]
Tong, Qunyi [2 ]
机构
[1] Hefei Normal Univ, Dept Life Sci, Hefei 230061, Anhui, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China
关键词
Gellan gum; Hydrogen peroxide; Sphingomonas paucimobilis; Oxidative stresses; FERMENTATIVE PRODUCTION; UDPG-PYROPHOSPHORYLASE; ATCC-31461; OPTIMIZATION; IMPROVEMENT; PH;
D O I
10.1007/s00449-013-1030-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this study, the effect of H2O2-induced oxidative stress on gellan gum production and cell growth were investigated. Gellan gum production was improved and cell growth was inhibited by H2O2. A multiple H2O2 stresses with different concentrations were developed to optimize gellan gum production. A maximal gellan gum yield (22.52 g/L), which was 35.58 % higher than the control, was observed with 2, 2, 3, 4 mmol/L H2O2 added at 6, 12, 18, 24 h, respectively. Moreover, UDP-glucose pyrophosphorylase activity and glucosyltransferase activity were increased with H2O2 stresses. This new strategy of multiple H2O2-induced oxidative stresses would be further applied to gellan gum production in future study.
引用
收藏
页码:743 / 748
页数:6
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