LncRNA PVT1 promotes cells proliferation via PI3K-AKT-mTOR Pathway in gastrointestinal stromal tumor

Gastrointestinal stromal tumor (GIST) is one of the most common mesenchymal tumors of the gastrointestinal tract by activating mutations in the related receptor tyrosine kinases KIT and PDGFRA. Few reliable biomarkers have been established despite illustration of the molecular mechanisms of GISTs development. Accumulating evidence has demonstrated that long non-coding RNA (lncRNAs) plasmacytoma variant translocation 1 (lncRNA PVT1) is a tumor regulator in many cancers. The goal of our research was to investigate the level and biological function of PVT1 in GIST-882 and GIST-T1 cells. The relative expression of PVT1 was higher in the GISTs cells. Down-regulation of PVT1 has shown that suppressed the proliferation and migration abilities of GISTs cells, and significantly stimulated cell apoptosis. Furthermore, western blot assay confirmed that knockdown of PVT1 obviously reduced the expression of CCND1, CCNB1, CDK6, PIK3CA, p-AKT, Rictor, mSIN1 and mLST8. The results implicated that PTV1 may regulate the progression of GISTs cells through PI3K/AKT/mTOR pathway. In conclusion, our study provides the evidence that PVT1 plays a vital oncogenic role in GISTs cells, and is a potential prognostic biomarker of recurrence after surgical procedures of GISTs. This highlights the possible of PVT1 serve as a therapeutic target for GISTs treatment.