RETRACTED: Long non-coding RNA MEG3 silencing and microRNA-214 restoration elevate osteoprotegerin expression to ameliorate osteoporosis by limiting TXNIP (Retracted article. See NOV, 2022)

被引:14
作者
Yang, ChangSheng [1 ]
Gu, ZhengTao [2 ]
Ding, Rui [1 ]
Huang, CaiQiang [1 ]
Li, QingChu [3 ]
Xie, DengHui [1 ]
Zhang, RongKai [1 ]
Qiu, YiYan [1 ]
机构
[1] Southern Med Univ, Guangdong Prov Key Lab Bone & Joint Degenerat Dis, Acad Orthoped Guangdong Prov, Div Spine Surg,Dept Orthoped,Affiliated Hosp 3, Sect 2,183 Zhongshan Ave West, Guangzhou 510630, Guangdong, Peoples R China
[2] Southern Med Univ, Guangdong Prov Key Lab Bone & Joint Degenerat Dis, Acad Orthoped Guangdong Prov,Dept Treatment, Ctr Traumat Injuries,Affifiliated Hosp 3, Guangzhou, Peoples R China
[3] Southern Med Univ, Guangdong Prov Key Lab Bone & Joint Degenerat Dis, Acad Orthoped Guangdong Prov, Div Joint Surg,Dept Orthoped,Affiliated Hosp 3, Guangzhou, Peoples R China
基金
美国国家科学基金会;
关键词
differentiation; long non‐ coding RNA maternally expressed gene 3; microRNA‐ 214; osteoblasts; osteoporosis; osteoprotegerin; proliferation; thioredoxin‐ interacting protein; POSTMENOPAUSAL OSTEOPOROSIS; OSTEOGENIC DIFFERENTIATION; PROMOTES APOPTOSIS; CANCER; CELLS; PROLIFERATION; INVASION;
D O I
10.1111/jcmm.16096
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Studies have shown that long non-coding RNA (lncRNA) MEG3 plays a key role in osteoporosis (OP), but its regulatory mechanism is somewhat incompletely clear. Here, we intend to probe into the mechanism of MEG3 on OP development by modulating microRNA-214 (miR-214) and thioredoxin-interacting protein (TXNIP). Rat models of OP were established. MEG3, miR-214 and TXNIP mRNA expression in rat femoral tissues were detected, along with TXNIP, OPG and RANKL protein expression. BMD, BV/TV, Tb.N and Tb.Th in tissue samples were measured. Ca, P and ALP contents in rat serum were also determined. Primary osteoblasts were isolated and cultured. Viability, COL-I, COL-II and COL-X mRNA expression, PCNA, cyclin D1, OCN, RUNX2 and osteolix protein expresion, ALP content and activity, and mineralized nodule area of rat osteoblasts were further detected. Dual-luciferase reporter gene and RNA-pull down assays verified the targeting relationship between MEG3, miR-214 and TXNIP. MEG3 and TXNIP were up-regulated while miR-214 was down-regulated in femoral tissues of OP rats. MEG3 silencing and miR-214 overexpression increased BMD, BV/TV, Tb.N, Tb.Th, trabecular bone area, collagen area and OPG expression, and down-regulated RANKL of femoral tissues in OP rats. MEG3 silencing and miR-214 overexpression elevated Ca and P and reduced ALP in OP rat serum, elevated osteoblast viability, differentiation ability, COL-I and COL-X expression and ALP activity, and reduced COL-II expression of osteoblasts. MEG3 specifically bound to miR-214 to regulate TXNIP. MEG3 silencing and miR-214 overexpression promote proliferation and differentiation of osteoblasts in OP by down-regulating TXNIP, which further improves OP.
引用
收藏
页码:2025 / 2039
页数:15
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