Inhibition of KCNQ1-4 potassium channels expressed in mammalian cells via M1 muscarinic acetylcholine receptors

被引:143
作者
Selyanko, AA
Hadley, JK
Wood, IC
Abogadie, FC
Jentsch, TJ
Brown, DA
机构
[1] UCL, Dept Pharmacol, London WC1E 6BT, England
[2] UCL, Wellcome Lab Mol Pharmacol, London WC1E 6BT, England
[3] Univ Hamburg, ZMNH, D-20246 Hamburg, Germany
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2000年 / 522卷 / 03期
关键词
D O I
10.1111/j.1469-7793.2000.t01-2-00349.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. KCNQ1-4 potassium channels were expressed in mammalian Chinese hamster ovary (CHO) cells stably transfected with M(1) muscarinic acetylcholine receptors and currents were recorded using the whole-cell perforated patch technique and cell-attached patch recording. 2. Stimulation of M(1) receptors by 10 mu M oxotremorine-M (Oxo-M) strongly reduced (to 0-10%) currents produced by KCNQ1-4 subunits expressed individually and also those produced by KCNQ2+KCNQ3 and KCNQ1+KCNE1 heteromers, which are thought to generate neuronal M-currents (I(K,M)) and cardiac slow delayed rectifier currents (I(K,s)), respectively. 3. The activity of KCNQ2+KCNQ3, KCNQ2 and KCNQ3 channels recorded with cell-attached pipettes was strongly and reversibly reduced by Oxo-M applied to the extra-patch membrane. 4. It is concluded that M, receptors couple to all known KCNQ subunits and that inhibition of KCNQ2+KCNQ3 channels, like that of native M-channels, requires a diffusible second messenger.
引用
收藏
页码:349 / 355
页数:7
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